Slow Cardiac Myosin Regulatory Light Chain 2 (MYL2) was Down-Expressed in Chronic Heart Failure Patients

Authors

  • Yuanhong Li,

    1. Department of Cardiology (Li, Wu, Tang, C. Huang, Jiang), Renmin Hospital of Wuhan University, Wuhan, People's Republic of China; Department of Cardiology (Li, J. Huang, Zhu, Wang), Central Hospital of Enshi Autonomous Prefecture, Enshi, People's Republic of China; Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research (Shi, Tu), Huazhong University of Science and Technology, Wuhan, People's Republic of China
    Search for more papers by this author
  • Gang Wu,

    1. Department of Cardiology (Li, Wu, Tang, C. Huang, Jiang), Renmin Hospital of Wuhan University, Wuhan, People's Republic of China; Department of Cardiology (Li, J. Huang, Zhu, Wang), Central Hospital of Enshi Autonomous Prefecture, Enshi, People's Republic of China; Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research (Shi, Tu), Huazhong University of Science and Technology, Wuhan, People's Republic of China
    Search for more papers by this author
  • Qizhu Tang, MD Qizhu Tang,

    Corresponding author
    1. Department of Cardiology (Li, Wu, Tang, C. Huang, Jiang), Renmin Hospital of Wuhan University, Wuhan, People's Republic of China; Department of Cardiology (Li, J. Huang, Zhu, Wang), Central Hospital of Enshi Autonomous Prefecture, Enshi, People's Republic of China; Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research (Shi, Tu), Huazhong University of Science and Technology, Wuhan, People's Republic of China
    • Renmin Hospital of Wuhan University Ziyang Road 95 Wuhan 430060, People's Republic of China.
    Search for more papers by this author
  • Congxin Huang,

    1. Department of Cardiology (Li, Wu, Tang, C. Huang, Jiang), Renmin Hospital of Wuhan University, Wuhan, People's Republic of China; Department of Cardiology (Li, J. Huang, Zhu, Wang), Central Hospital of Enshi Autonomous Prefecture, Enshi, People's Republic of China; Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research (Shi, Tu), Huazhong University of Science and Technology, Wuhan, People's Republic of China
    Search for more papers by this author
  • Hong Jiang,

    1. Department of Cardiology (Li, Wu, Tang, C. Huang, Jiang), Renmin Hospital of Wuhan University, Wuhan, People's Republic of China; Department of Cardiology (Li, J. Huang, Zhu, Wang), Central Hospital of Enshi Autonomous Prefecture, Enshi, People's Republic of China; Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research (Shi, Tu), Huazhong University of Science and Technology, Wuhan, People's Republic of China
    Search for more papers by this author
  • Lisong Shi,

    1. Department of Cardiology (Li, Wu, Tang, C. Huang, Jiang), Renmin Hospital of Wuhan University, Wuhan, People's Republic of China; Department of Cardiology (Li, J. Huang, Zhu, Wang), Central Hospital of Enshi Autonomous Prefecture, Enshi, People's Republic of China; Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research (Shi, Tu), Huazhong University of Science and Technology, Wuhan, People's Republic of China
    Search for more papers by this author
  • Xin Tu,

    1. Department of Cardiology (Li, Wu, Tang, C. Huang, Jiang), Renmin Hospital of Wuhan University, Wuhan, People's Republic of China; Department of Cardiology (Li, J. Huang, Zhu, Wang), Central Hospital of Enshi Autonomous Prefecture, Enshi, People's Republic of China; Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research (Shi, Tu), Huazhong University of Science and Technology, Wuhan, People's Republic of China
    Search for more papers by this author
  • Jinqi Huang,

    1. Department of Cardiology (Li, Wu, Tang, C. Huang, Jiang), Renmin Hospital of Wuhan University, Wuhan, People's Republic of China; Department of Cardiology (Li, J. Huang, Zhu, Wang), Central Hospital of Enshi Autonomous Prefecture, Enshi, People's Republic of China; Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research (Shi, Tu), Huazhong University of Science and Technology, Wuhan, People's Republic of China
    Search for more papers by this author
  • Xiaoqing Zhu,

    1. Department of Cardiology (Li, Wu, Tang, C. Huang, Jiang), Renmin Hospital of Wuhan University, Wuhan, People's Republic of China; Department of Cardiology (Li, J. Huang, Zhu, Wang), Central Hospital of Enshi Autonomous Prefecture, Enshi, People's Republic of China; Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research (Shi, Tu), Huazhong University of Science and Technology, Wuhan, People's Republic of China
    Search for more papers by this author
  • Hongli Wang

    1. Department of Cardiology (Li, Wu, Tang, C. Huang, Jiang), Renmin Hospital of Wuhan University, Wuhan, People's Republic of China; Department of Cardiology (Li, J. Huang, Zhu, Wang), Central Hospital of Enshi Autonomous Prefecture, Enshi, People's Republic of China; Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research (Shi, Tu), Huazhong University of Science and Technology, Wuhan, People's Republic of China
    Search for more papers by this author

Abstract

Background

Genetic studies have shown that many slow cardiac myosin regulatory light chain 2 (MYL2) gene mutations can cause hypertrophic cardiomyopathy, which is one of the most common causes of heart failure (HF). But until now there has been no pathological or histological evidence that MYL2 may be associated with HF development. Recent microarray studies indicated that myosin heavy chain expression changed in the pathological process of HF. Because MYL2 is a regulatory component of myosin heavy polypeptide, the role of MYL2 protein in HF needs to be studied.

Hypothesis

The level of expression of MYL2 may change in the heart tissue of patients with chronic HF.

Methods

We collected 28 human right auricle samples, 16 from chronic HF patients and 12 from healthy control subjects. Immunohistochemistry was carried out to observe the tissue-expression pattern of the MYL2 protein and Western blot methods were performed to quantify the relative MYL2 expression level.

Results

In chronic HF patients, the MYL2 protein level decreased significantly compared with normal controls (t test P < 0.05). Among the 16 HF patients, MYL2 expression in the severe HF group (New York Heart Association [NYHA] class III or IV) was even lower than that of the moderate HF group (NYHA class II) (t test P < 0.05).

Conclusions

MYL2 was down-expressed in HF tissues, and our findings suggested that MYL2 may play a role in the development and progression of chronic HF. Copyright © 2008 Wiley Periodicals, Inc.

The first two authors contributed equally to this work. This study was supported by the National Natural Science Foundation of China (30800457, 30900803) and the Natural Science Program of Hubei Province (2009CDZ013). This study has no relationship with any kind of industry. The authors have no other funding, financial relationships, or conflicts of interest to disclose.

Ancillary