Screening for and Identification of an Anti-clam Vibrio Marine Bacterium from an Aquaculture Pond in the Yellow Sea

Authors

  • Xing Sun,

    1. Jiangsu Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing Jiangsu Province, Nanjing, P .R. China
    2. Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai Shandong, P. R. China
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    • These authors contributed equally to this work.
  • Gengmao Zhao,

    Corresponding author
    1. Jiangsu Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing Jiangsu Province, Nanjing, P .R. China
    • Correspondence: Professor Jiangsu Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing Jiangsu Province 1 Tongwei Road, Nanjing, 210095, P. R. China

      E-mail: zhaogengmao@126.com

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  • Chenhao Gu,

    1. College of life Science, Nanjing Agricultural University, Nanjing Jiangsu Province, Nanjing, P. R. China
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    • These authors contributed equally to this work.
  • Ling Liu,

    1. Jiangsu Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing Jiangsu Province, Nanjing, P .R. China
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    • These authors contributed equally to this work.
  • Ming Zhu,

    1. Jiangsu Key Laboratory of Marine Biotechnology, Huai Hai Institute of Technology, Lianyungang Jiangsu Province, Lianyungang, P. R. China
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  • Zhaopu Liu

    1. Jiangsu Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing Jiangsu Province, Nanjing, P .R. China
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Abstract

The identification and use of probiotic bacterial stains is a practical approach to protect clams grown in aquaculture farms from disease. The inhibition of the pathogenic bacterium Vibrio alginolyticus was used as a trait to select a candidate probiotic bacterial strain in this study. An ideal bacterial strain, SW-1, was isolated from seawater from a clam farm. The selected isolate SW-1 was identified based on its physiological, morphological, and biochemical characteristics and its 16S rDNA sequence. The experiments showed that strain SW-1 had a high similarity to Pseudoalteromonas piscicida and could inhibit the growth of V. alginolyticus (V.-MP-1). SW-1 also improved the survival of clams following challenge with the pathogenic V.-MP-1. The mortality of clams was 100% after infection with 108 CFU/mL of V. alginolyticus, whereas mortality was only 11% when clams were infected with 108 CFU/mL of V.-MP1 while simultaneously exposed to the same concentration of Pseudoalteromonas SW-1, indicating that Pseudoalteromonas SW-1 could be used as a probiotic to protect farmed clams, and thus reduce the effects of antibiotics on aquatic environment.

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