Causal mapping as a tool to mechanistically interpret phenomena in cell motility: Application to cortical oscillations in spreading cells
Article first published online: 23 JUN 2006
Copyright © 2006 Wiley-Liss, Inc.
Cell Motility and the Cytoskeleton
Volume 63, Issue 9, pages 523–532, September 2006
How to Cite
Weinreb, G. E., Elston, T. C. and Jacobson, K. (2006), Causal mapping as a tool to mechanistically interpret phenomena in cell motility: Application to cortical oscillations in spreading cells. Cell Motil. Cytoskeleton, 63: 523–532. doi: 10.1002/cm.20143
- Issue published online: 10 AUG 2006
- Article first published online: 23 JUN 2006
- Manuscript Accepted: 12 MAY 2006
- Manuscript Received: 16 FEB 2006
- NIH Cell Migration Consortium. Grant Number: GM64346
- NIH. Grant Numbers: GM41402, GM073180, GM078994
- systems biology;
- cell motility;
Biological processes that occur at the cellular level and consist of large numbers of interacting elements are highly nonlinear and generally involve multiple time and spatial scales. The quantitative description of these complex systems is of great importance but presents large challenges. We outline a new systems biology approach, causal mapping (CMAP), which is a coarse-grained biological network tool that permits description of causal interactions between the elements of the network and overall system dynamics. On one hand, the CMAP is an intermediate between experiments and physical modeling, describing major requisite elements, their interactions and paths of causality propagation. On the other hand, the CMAP is an independent tool to explore the hierarchical organization of cell and the role of uncertainties in the system. It appears to be a promising easy-to-use technique for cell biologists to systematically probe verbally formulated qualitative hypotheses. We apply the CMAP to study the phenomenon of contractility oscillations in spreading cells in which microtubules have been depolymerized. The precise mechanism by which these oscillations are governed by a complex mechano-chemical system is not known but the data observed in experiments can be described by a CMAP. The CMAP suggests that the source of the oscillations results from the opposing effects of Rho activation leading to a decreased level of myosin light chain phosphatase and a cyclic calcium influx caused by increased membrane tension and leading to a periodically enhanced activation of myosin light chain kinase. Cell Motil. Cytoskeleton 2006. © 2006 Wiley-Liss, Inc.