Caveolin-1 polarization in migrating endothelial cells is directed by substrate topology not chemoattractant gradient
Article first published online: 7 SEP 2006
Copyright © 2006 Wiley-Liss, Inc.
Cell Motility and the Cytoskeleton
Volume 63, Issue 11, pages 673–680, November 2006
How to Cite
Santilman, V., Baran, J., Anand-Apte, B., Fox, P. L. and Parat, M.-O. (2006), Caveolin-1 polarization in migrating endothelial cells is directed by substrate topology not chemoattractant gradient. Cell Motil. Cytoskeleton, 63: 673–680. doi: 10.1002/cm.20153
- Issue published online: 11 OCT 2006
- Article first published online: 7 SEP 2006
- Manuscript Accepted: 26 JUL 2006
- Manuscript Received: 4 NOV 2005
- American Heart Association. Grant Numbers: 0525466B, 0235057N
- Foundation Fighting Blindness
- NIH. Grant Numbers: R01 HL75255, R01 CA106415, R01 EY016490
- cell migration;
Polarization is a hallmark of migrating cells, and an asymmetric distribution of proteins is essential to the migration process. Caveolin-1 is highly polarized in migrating endothelial cells (EC). Several studies have shown caveolin-1 accumulation in the front of migrating EC while others report its accumulation in the EC rear. In this paper we address these conflicting results on polarized localization of caveolin-1. We find evidence for the hypothesis that different modes of locomotion lead to differences in protein polarization. In particular, we show that caveolin-1 is primarily localized in the rear of cells migrating on a planar substrate, but in the front of cells traversing a three-dimensional pore. We also show that a chemoattractant, present either as a gradient or ubiquitously in the medium, does not alter caveolin-1 localization in cells in either mode of locomotion. Thus we conclude that substrate topology, and not the presence of a chemoattractant, directs the polarization of caveolin-1 in motile ECs. Cell Motil. Cytoskeleton 2006. © 2006 Wiley-Liss, Inc.