Calcium-dependent flagellar motility activation in Chlamydomonas reinhardtii in response to mechanical agitation
Article first published online: 18 JUN 2009
Copyright © 2009 Wiley-Liss, Inc.
Cell Motility and the Cytoskeleton
Volume 66, Issue 9, pages 736–742, September 2009
How to Cite
Wakabayashi, K.-i., Ide, T. and Kamiya, R. (2009), Calcium-dependent flagellar motility activation in Chlamydomonas reinhardtii in response to mechanical agitation. Cell Motil. Cytoskeleton, 66: 736–742. doi: 10.1002/cm.20402
- Issue published online: 5 AUG 2009
- Article first published online: 18 JUN 2009
- Manuscript Accepted: 27 MAY 2009
- Manuscript Received: 30 MAR 2009
- Grant-in-Aid for Young Scientists (Start-up and B)
- Japan Society for Promotion of Sciences
- Asahi Glass Foundation
- Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology
Flagellar beating in Chlamydomonas was found to be activated by mechanical stimulation. Immediately after a wild-type cell suspension was vortexed, the average swimming velocity of cells increased from 130 μm/second to 150 μm/second, due to an elevation of flagellar beat frequency from ∼60 Hz to ∼70 Hz without detectable change in the flagellar waveforms. This response required outer arm dynein. Treatment with EGTA, Ca2+-channel blockers, or mechanosensitive-channel blockers inhibited it. In demembranated and reactivated cell models, a modest increase in Ca2+ concentration elevated the axonemal beat frequency. These data indicate that the mechanical agitation increases beat frequency because it causes Ca2+ influx into flagella, which then activates outer arm dynein. Cell Motil. Cytoskeleton 2009. © 2009 Wiley-Liss, Inc.