Matthew B. Smith, Hongsheng Li, and Tian Shen contributed equally to this work.
Segmentation and tracking of cytoskeletal filaments using open active contours†
Article first published online: 7 OCT 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 67, Issue 11, pages 693–705, November 2010
How to Cite
Smith, M. B., Li, H., Shen, T., Huang, X., Yusuf, E. and Vavylonis, D. (2010), Segmentation and tracking of cytoskeletal filaments using open active contours. Cytoskeleton, 67: 693–705. doi: 10.1002/cm.20481
Monitoring Editor: Bruce Goode
- Issue published online: 19 OCT 2010
- Article first published online: 7 OCT 2010
- Accepted manuscript online: 2 SEP 2010 07:01AM EST
- Manuscript Accepted: 20 AUG 2010
- Manuscript Revised: 19 AUG 2010
- Manuscript Received: 26 APR 2010
- NIH Grant. Grant Number: R21GM083928
- Biosystems Dynamics Summer Institute at Lehigh University
- active contours
We use open active contours to quantify cytoskeletal structures imaged by fluorescence microscopy in two and three dimensions. We developed an interactive software tool for segmentation, tracking, and visualization of individual fibers. Open active contours are parametric curves that deform to minimize the sum of an external energy derived from the image and an internal bending and stretching energy. The external energy generates (i) forces that attract the contour toward the central bright line of a filament in the image, and (ii) forces that stretch the active contour toward the ends of bright ridges. Images of simulated semiflexible polymers with known bending and torsional rigidity are analyzed to validate the method. We apply our methods to quantify the conformations and dynamics of actin in two examples: actin filaments imaged by TIRF microscopy in vitro, and actin cables in fission yeast imaged by spinning disk confocal microscopy. © 2010 Wiley-Liss, Inc.