F-actin distribution at nodes of Ranvier and Schmidt-Lanterman incisures in mammalian sciatic nerves

Authors

  • Alejandra Kun,

    1. Department of Proteins and Nucleic Acids, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Av. Italia 3318, Montevideo, Uruguay
    2. Department of Biochemistry, School of Science, Universidad de la República (UdelaR), Montevideo, Uruguay
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  • Lucía Canclini,

    1. Department of Proteins and Nucleic Acids, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Av. Italia 3318, Montevideo, Uruguay
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  • Gonzalo Rosso,

    1. Department of Proteins and Nucleic Acids, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Av. Italia 3318, Montevideo, Uruguay
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  • Mariana Bresque,

    1. Department of Proteins and Nucleic Acids, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Av. Italia 3318, Montevideo, Uruguay
    2. Project of the Comisión Sectorial de Investigación Científica (CSIC, UdelaR), Montevideo, Uruguay
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  • Carlos Romeo,

    1. Department of Proteins and Nucleic Acids, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Av. Italia 3318, Montevideo, Uruguay
    2. Project of the Comisión Sectorial de Investigación Científica (CSIC, UdelaR), Montevideo, Uruguay
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  • Alicia Hanusz,

    1. Department of Proteins and Nucleic Acids, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Av. Italia 3318, Montevideo, Uruguay
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  • Karina Cal,

    1. Department of Proteins and Nucleic Acids, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Av. Italia 3318, Montevideo, Uruguay
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  • Aldo Calliari,

    1. Department of Proteins and Nucleic Acids, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Av. Italia 3318, Montevideo, Uruguay
    2. Area Biofísica, School of Veterinary, UdelaR, Montevideo, Uruguay
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  • José Sotelo Silveira,

    1. Department of Cell and Molecular Biology, School of Science, UdelaR, Montevideo, Uruguay
    2. Department of Genetics, IIBCE, Montevideo, Uruguay
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  • José R. Sotelo

    Corresponding author
    1. Department of Proteins and Nucleic Acids, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Av. Italia 3318, Montevideo, Uruguay
    • Head of the Department of Proteins and Nucleic Acids, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Av. Italia 3318, Montevideo, Uruguay
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  • Monitoring Editor: Peter Baas

Abstract

Very little is known about the function of the F-actin cytoskeleton in the regeneration and pathology of peripheral nerve fibers. The actin cytoskeleton has been associated with maintenance of tissue structure, transmission of traction and contraction forces, and an involvement in cell motility. Therefore, the state of the actin cytoskeleton strongly influences the mechanical properties of cells and intracellular transport therein. In this work, we analyze the distribution of F-actin at Schmidt-Lanterman Incisures (SLI) and nodes of Ranvier (NR) domains in normal, regenerating and pathologic Trembler J (TrJ/+) sciatic nerve fibers, of rats and mice. F-actin was quantified and it was found increased in TrJ/+, both in SLI and NR. However, SLI and NR of regenerating rat sciatic nerve did not show significant differences in F-actin, as compared with normal nerves. Cytochalasin-D and Latrunculin-A were used to disrupt the F-actin network in normal and regenerating rat sciatic nerve fibers. Both drugs disrupt F-actin, but in different ways. Cytochalasin-D did not disrupt Schwann cell (SC) F-actin at the NR. Latrunculin-A did not disrupt F-actin at the boundary region between SC and axon at the NR domain. We surmise that the rearrangement of F-actin in neurological disorders, as presented here, is an important feature of TrJ/+ pathology as a Charcot-Marie-Tooth (CMT) model. © 2012 Wiley Periodicals, Inc

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