Monitoring Editor: Peter Baas
Dyrk kinases regulate phosphorylation of doublecortin, cytoskeletal organization, and neuronal morphology†
Version of Record online: 7 MAR 2012
Copyright © 2012 Wiley Periodicals, Inc.
Special Issue: Emerging Concepts in Neuronal Cytoskeleton
Volume 69, Issue 7, pages 514–527, July 2012
How to Cite
Slepak, T. I., Salay, L. D., Lemmon, V. P. and Bixby, J. L. (2012), Dyrk kinases regulate phosphorylation of doublecortin, cytoskeletal organization, and neuronal morphology. Cytoskeleton, 69: 514–527. doi: 10.1002/cm.21021
- Issue online: 6 JUL 2012
- Version of Record online: 7 MAR 2012
- Accepted manuscript online: 22 FEB 2012 12:32PM EST
- Manuscript Accepted: 16 FEB 2012
- Manuscript Revised: 15 FEB 2012
- Manuscript Received: 1 NOV 2011
Additional Supporting Information may be found in the online version of this article.
|CM_21021_sm_SuppFig1.pdf||814K||Supporting Information Fig.1. Dyrk2 affects subcellular localization of endogenous DCX in dendrites and axons. Neurons were co-transfected with Dyrk2 and a GFP expressing plasmid, and stained for DCX (red) and β3 tubulin (white). A cell expressing Dyrk2 is marked by the arrow on panel A. Magnified images of the axon (inset a) and dendrites (inset b) from the transfected neuron are shown on the right (B, C and D, E, respectively). The arrows point to the axon and dendrites in the transfected neuron, and the arrowheads to the untransfected neuron.|
|CM_21021_sm_SuppFig2.pdf||448K||Supporting Information Fig. 2. Dyrk2 overexpression abolishes the effect of the DCX S306A mutant on axonal curviness. A. Hippocampal neurons were transfected with plasmids expressing 2A-tagged DCX S306A together with mCherry (mCh, control) or Dyrk2 (D2). Cells were stained with anti-2A and anti-?beta;3 tubulin antibodies. Arrows mark transfected cells. The mutant DCX produces long and curvy neurites, and expression of Dyrk2 strongly reduces this curviness. B. Average tortuosity was measured as in Figure 6 (Mean ± SEM) for axons (black bars) and dendrites (white bars), respectively. DCX S306A overexpression significantly increased tortuosity of axons but not dendrites. Co-expression of Dyrk2 reduced the tortuosity to that of control. All neurites were measured for N≥20 cells. ***; p< 0.0001.|
|CM_21021_sm_SuppTabI.pdf||26K||Supporting Information Table I. Summary of morphological parameters altered in neurons transfected with WT and KD Dyrks. Axon total length (Ax Lngth), dendrite total length (Dn Lngth), number of axonal branches (Ax Br), and number of dendritic branches (Dn Br) were each normalized to values for control transfected cells and expressed as a percentage (Mean ± SEM). Data were collected from 2 independent experiments (N≥30 cells) and analyzed using one-way Analysis of Variance (ANOVA), Dunnett's Multiple Comparison Test.|
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