SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
CM_21021_sm_SuppFig1.pdf814KSupporting Information Fig.1. Dyrk2 affects subcellular localization of endogenous DCX in dendrites and axons. Neurons were co-transfected with Dyrk2 and a GFP expressing plasmid, and stained for DCX (red) and β3 tubulin (white). A cell expressing Dyrk2 is marked by the arrow on panel A. Magnified images of the axon (inset a) and dendrites (inset b) from the transfected neuron are shown on the right (B, C and D, E, respectively). The arrows point to the axon and dendrites in the transfected neuron, and the arrowheads to the untransfected neuron.
CM_21021_sm_SuppFig2.pdf448KSupporting Information Fig. 2. Dyrk2 overexpression abolishes the effect of the DCX S306A mutant on axonal curviness. A. Hippocampal neurons were transfected with plasmids expressing 2A-tagged DCX S306A together with mCherry (mCh, control) or Dyrk2 (D2). Cells were stained with anti-2A and anti-?beta;3 tubulin antibodies. Arrows mark transfected cells. The mutant DCX produces long and curvy neurites, and expression of Dyrk2 strongly reduces this curviness. B. Average tortuosity was measured as in Figure 6 (Mean ± SEM) for axons (black bars) and dendrites (white bars), respectively. DCX S306A overexpression significantly increased tortuosity of axons but not dendrites. Co-expression of Dyrk2 reduced the tortuosity to that of control. All neurites were measured for N≥20 cells. ***; p< 0.0001.
CM_21021_sm_SuppTabI.pdf26KSupporting Information Table I. Summary of morphological parameters altered in neurons transfected with WT and KD Dyrks. Axon total length (Ax Lngth), dendrite total length (Dn Lngth), number of axonal branches (Ax Br), and number of dendritic branches (Dn Br) were each normalized to values for control transfected cells and expressed as a percentage (Mean ± SEM). Data were collected from 2 independent experiments (N≥30 cells) and analyzed using one-way Analysis of Variance (ANOVA), Dunnett's Multiple Comparison Test.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.