T.G.C. and C.L. contributed equally to this work
Symmetric cell division of the mouse zygote requires an actin network †
Article first published online: 28 AUG 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 69, Issue 12, pages 1040–1046, December 2012
How to Cite
Chew, T. G., Lorthongpanich, C., Ang, W. X., Knowles, B. B. and Solter, D. (2012), Symmetric cell division of the mouse zygote requires an actin network . Cytoskeleton, 69: 1040–1046. doi: 10.1002/cm.21062
Monitoring Editor: Roberto Dominguez
- Issue published online: 12 DEC 2012
- Article first published online: 28 AUG 2012
- Accepted manuscript online: 6 AUG 2012 02:36PM EST
- Manuscript Accepted: 30 JUL 2012
- Manuscript Received: 17 MAY 2012
- Agency for Science, Technology and Research (A*STAR) Singapore
Additional Supporting Information may be found in the online version of this article.
|CM_21062_sm_SuppFig1.jpg||1336K||Figure S1. Cytoplasmic F-actin assembles into a cloud-like structure during the first mitosis. Samples were processed as in Figure 1, except embryos were permeabilized for 30 minutes, and no blocking solution or antibodies were added. The actin network was detected by Alex Fluor 488 phalloidin. Arrows indicate the actin cloud. Scale bar represents 50µm.|
|CM_21062_sm_SuppFig2.jpg||533K||Figure S2. The actin network does not show much of the co-localization with the nuclear membrane protein Lamin B. The mouse zygote was stained with Alexa Fluor-conjugated phalloidin and Lamin B antibody to reveal the actin network and the pronuclear membrane. Scale bar represents 50µm.|
|CM_21062_sm_SuppFig3.jpg||1905K||Figure S3. Spindle relocation procedure. Centrally positioned mitotic spindles were visualized using the Oosight Spindle View Imaging System and were micromanipulated to the sub-cortical region using a micropipette controlled by a micromanipulator (I to IV).|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.