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CM_21079_sm_SuppVideo1.avi96KMovie S1. Golgi Microtubule Nucleation in Prophase Cells LLC-PK1 cells stably expressing GFP-EB1 (green) were transfected with mCherry-Rab6 construct (red) and imaged by wide field epifluorescence microscopy. The movie illustrates that Golgi fragments retain their ability to nucleate MTs during prophase. A zoom of the boxed area is represented in the right panel to highlight the EB1 comet originated at the Golgi (arrow). Five seconds between frames.
CM_21079_sm_SuppVideo2.avi135KMovie S2. Golgi Derived Microtubules are Present during Anaphase LLC-PK1 cells stably expressing GFP-EB1 (green) were transfected with mCherry-Rab6 construct (red) and imaged by wide field epifluorescence microscopy. The movie illustrates that Golgi fragments retain their ability to nucleate MTs during anaphase. A zoom of the boxed area is represented in the right panel to highlight the EB1 comet originated at the Golgi (arrow). Five seconds between frames.
CM_21079_sm_SuppVideo3.avi935KMovie S3. Telophase Cells Nucleate Microtubules from the Golgi Stacks; example 1 LLC-PK1 cells stably expressing GFP-EB1 (green) were transfected with mCherry-Rab6 construct (red) and imaged by wide field epifluorescence microscopy. The movie illustrates that Golgi stacks are able to nucleated MTs during telophase. A zoom of the boxed area is represented in the right panel to highlight the EB1 comet originated at the Golgi (arrow). Five seconds between frames.
CM_21079_sm_SuppVideo4.avi818KMovie S4. Telophase Cells Nucleate Microtubules from the Golgi Stacks; example 2 LLC-PK1 cells stably expressing GFP-EB1 (green) were transfected with mCherry-Rab6 construct (red), imaged by wide field epifluorescence microscopy, and then deconvolved. The movie illustrates that Golgi stacks are able to nucleated MTs during telophase. A zoom of the boxed area is represented in the right panel to highlight the EB1 comet originated at the Golgi (arrow). Five seconds between frames.
CM_21079_sm_SuppVideo5.avi634KMovie S5. Golgi assembly in an LLCPPK cell expressing RFP-TGN (false-colored green) at mitotic exit, visualized by spinning disk confocal microscopy. Maximal projection of confocal z stacks is shown. Golgi complex rapidly assembles into an integral complex, which thereafter remains a single entity despite dynamic shape change Arrow appears at approximate time of complex assembly. Time, minutes.
CM_21079_sm_SuppVideo6.avi730KMovie S6. Golgi assembly in an LLCPPK cell expressing GFP-AKAPdn (not shown) and RFP-TGN (false-colored green) at mitotic exit, visualized by spinning disk confocal microscopy. Maximal projection of confocal z stacks is shown. Golgi complex assembly is slow and incomplete even 55 minutes of assembly. Arrow indicates time point, at which the control Golgi formed a single complex in Movie S5. Time, minutes.

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