Monitoring Editor: Miklós Nyitrai
Probing cytoskeletal structures by coupling optical superresolution and AFM techniques for a correlative approach
Article first published online: 2 OCT 2013
Copyright © 2013 Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution-NonCommercialNoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is noncommercial and no modifi-cations or adaptations are made.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Special Issue: Biophysical Approaches for Investigation of the Cytoskeleton. Part II
Volume 70, Issue 11, pages 729–740, November 2013
How to Cite
Chacko, J. V., Zanacchi, F. C. and Diaspro, A. (2013), Probing cytoskeletal structures by coupling optical superresolution and AFM techniques for a correlative approach. Cytoskeleton, 70: 729–740. doi: 10.1002/cm.21139
- Issue published online: 25 NOV 2013
- Article first published online: 2 OCT 2013
- Accepted manuscript online: 12 SEP 2013 04:12AM EST
- Manuscript Accepted: 1 SEP 2013
- Manuscript Revised: 28 AUG 2013
- Manuscript Received: 22 JUL 2013
- Italian Programmi di ricerca di rilevante interesse nazionale. Grant Number: 2010JFYFY2_002
- STED AFM;
- correlative microscopy;
- STORM AFM
In this article, we describe and show the application of some of the most advanced fluorescence superresolution techniques, STED AFM and STORM AFM microscopy towards imaging of cytoskeletal structures, such as microtubule filaments. Mechanical and structural properties can play a relevant role in the investigation of cytoskeletal structures of interest, such as microtubules, that provide support to the cell structure. In fact, the mechanical properties, such as the local stiffness and the elasticity, can be investigated by AFM force spectroscopy with tens of nanometers resolution. Force curves can be analyzed in order to obtain the local elasticity (and the Young's modulus calculation by fitting the force curves from every pixel of interest), and the combination with STED/STORM microscopy integrates the measurement with high specificity and yields superresolution structural information. This hybrid modality of superresolution-AFM working is a clear example of correlative multimodal microscopy. © 2013 Wiley Periodicals, Inc.