Novel Forms of epithelial cell motility on collagen and on glass surfaces
Article first published online: 4 FEB 2005
Copyright © 1982 Wiley-Liss, Inc.
Volume 2, Issue 4, pages 333–341, 1982
How to Cite
Tchao, R. (1982), Novel Forms of epithelial cell motility on collagen and on glass surfaces. Cell Motility, 2: 333–341. doi: 10.1002/cm.970020403
- Issue published online: 4 FEB 2005
- Article first published online: 4 FEB 2005
- Manuscript Accepted: 20 APR 1982
- Manuscript Received: 1 DEC 1981
- cell motility;
The motility of an epithelial cell line NBT-II derived from a rat bladder tumor was examined on glass and on collagen. On glass the cells rotate in groups of 2–8 cells. Rotatory migration ceases as cells enter into mitosis; after mitosis, the daughter cells spread out and participate in the rotatory activity of the group. As the number of cells in a group increases the rate of rotatory migration slows, and groups with ten cells or more do not rotate consistently. On collagen NBT-II cells migrate as single cells in a smooth gliding fashion, with the broad lamellipodia as the leading front. After mitosis, the two daughter cells separate at 180° of each other and migrate away independently. Before totally spreading out on the collagen surface, the pair of daughter cells shows a characteristic twist of about 60° from their original position at telophase. The difference in motility of NBT II cells on glass and on collagen is explained in terms of differences in cell-to-cell cohesion and cell-to-substrate adhesion.