Analytic Techniques in Light Microscopy
Nanometer-scale measurements using video light microscopy
Article first published online: 4 FEB 2005
Copyright © 1988 Wiley-Liss, Inc.
Cell Motility and the Cytoskeleton
Volume 10, Issue 1-2, pages 47–53, 1988
How to Cite
Schnapp, B. J., Gelles, J. and Sheetz, M. P. (1988), Nanometer-scale measurements using video light microscopy. Cell Motil. Cytoskeleton, 10: 47–53. doi: 10.1002/cm.970100109
- Issue published online: 4 FEB 2005
- Article first published online: 4 FEB 2005
- Manuscript Accepted: 14 MAR 1988
- Manuscript Received: 19 FEB 1988
- motion analysis;
Video and digital image processing have been used to amplify the contrast of light microscopic images, making it possible to observe in real time the diffraction images of cell structures 10 times smaller than the Raleigh resolution limit of 0.2 μm. In this paper we discuss how quantitative analysis of diffraction images can be used to extract information about motion or structure at the nanometer level. This issue is considered in the context of a method for tracking the motion of kinesin-coated beads on microtubules with 1–2 nm precision (Gelles et al.: Nature 331:450–453, 1988).