These authors contributed equally to this work.
Evidence for the Existence of a Specific G Protein-Coupled Receptor Activated by Guanosine
Article first published online: 15 APR 2011
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 6, Issue 6, pages 1074–1080, June 6, 2011
How to Cite
Volpini, R., Marucci, G., Buccioni, M., Dal Ben, D., Lambertucci, C., Lammi, C., Mishra, R. C., Thomas, A. and Cristalli, G. (2011), Evidence for the Existence of a Specific G Protein-Coupled Receptor Activated by Guanosine. ChemMedChem, 6: 1074–1080. doi: 10.1002/cmdc.201100100
- Issue published online: 26 MAY 2011
- Article first published online: 15 APR 2011
- Manuscript Revised: 11 MAR 2011
- Manuscript Received: 17 FEB 2011
- Italian Ministry for University and Research. Grant Number: PRIN2008
- Ministry for Health. Grant Number: RF-CNM-2007-662855
Guanosine, released extracellularly from neurons and glial cells, plays important roles in the central nervous system, including neuroprotection. The innovative DELFIA Eu-GTP binding assay was optimized for characterization of the putative guanosine receptor binding site at rat brain membranes by using a series of novel and known guanosine derivatives. These nucleosides were prepared by modifying the purine and sugar moieties of guanosine at the 6- and 5′-positions, respectively. Results of these experiments prove that guanosine, 6-thioguanosine, and their derivatives activate a G protein-coupled receptor that is different from the well-characterized adenosine receptors.