Gadolinium- and Manganite-Based Contrast Agents with Fluorescent Probes for Both Magnetic Resonance and Fluorescence Imaging of Pancreatic Islets: A Comparative Study

Authors

  • Dr. Zuzana Berkova,

    1. Laboratory of Pancreatic Islets, Institute for Clinical and Experimental Medicine (IKEM), Videnska 1958/9, 14021 Prague (Czech Republic)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Dr. Daniel Jirak,

    Corresponding author
    1. Department of Diagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine (IKEM), Videnska 1958/9, 14021 Prague (Czech Republic)
    2. Institute of Biophysics and Informatics, 1st Medicine Faculty, Charles University, Salmovska 1, 12000 Prague (Czech Republic)
    • Department of Diagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine (IKEM), Videnska 1958/9, 14021 Prague (Czech Republic)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Dr. Klara Zacharovova,

    1. Laboratory of Pancreatic Islets, Institute for Clinical and Experimental Medicine (IKEM), Videnska 1958/9, 14021 Prague (Czech Republic)
    Search for more papers by this author
  • Prof. Ivan Lukes,

    1. Department of Inorganic Chemistry, Faculty of Science, Charles University, Hlavova 2030, 12843 Prague (Czech Republic)
    Search for more papers by this author
  • Dr. Zuzana Kotkova,

    1. Department of Inorganic Chemistry, Faculty of Science, Charles University, Hlavova 2030, 12843 Prague (Czech Republic)
    Search for more papers by this author
  • Dr. Jan Kotek,

    1. Department of Inorganic Chemistry, Faculty of Science, Charles University, Hlavova 2030, 12843 Prague (Czech Republic)
    Search for more papers by this author
  • Dr. Michal Kacenka,

    1. Department of Inorganic Chemistry, Faculty of Science, Charles University, Hlavova 2030, 12843 Prague (Czech Republic)
    Search for more papers by this author
  • Dr. Ondrej Kaman,

    1. Department of Cell Biology, Faculty of Science, Charles University, Albertov 6, 12843 Prague 2 (Czech Republic)
    2. Institute of Physics, Czech Academy of Sciences, Cukrovarnicka 10, 16200 Prague 6 (Czech Republic)
    Search for more papers by this author
  • Dr. Ivan Rehor,

    1. Department of Inorganic Chemistry, Faculty of Science, Charles University, Hlavova 2030, 12843 Prague (Czech Republic)
    Search for more papers by this author
  • Dr. Milan Hajek,

    1. Department of Diagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine (IKEM), Videnska 1958/9, 14021 Prague (Czech Republic)
    Search for more papers by this author
  • Prof. Frantisek Saudek

    1. Laboratory of Pancreatic Islets, Institute for Clinical and Experimental Medicine (IKEM), Videnska 1958/9, 14021 Prague (Czech Republic)
    Search for more papers by this author

Abstract

Three magnetic resonance (MR)/fluorescence imaging probes were tested for visualization, cellular distribution, and survival of labeled pancreatic islets in vitro and following transplantation. As T1 contrast agents (CAs), gadolinium(III) complexes linked to β-cyclodextrin (Gd-F-βCD) or bound to titanium dioxide (TiO2@RhdGd) were tested. As a T2 CA, perovskite manganite nanoparticles (LSMO@siF@si) were examined. Fluorescein or rhodamine was incorporated as a fluorescent marker in all probes. Islets labeled with gadolinium(III) CAs were visible as hyperintense spots on MR in vitro, but detection in vivo was inconclusive. Islets labeled with LSMO@siF@si CA were clearly visible as hypointense spots or areas on MR scans in vitro as well as in vivo. All CAs were detected inside the islet cells by fluorescence. Although the vitality and function of the labeled islets was not impaired by any of the tested CAs, results indicate that LSMO@siF@si CA is a superior marker for islet labeling, as it provides better contrast enhancement within a shorter scan time.

Ancillary