This article is a U.S. Government work and is in the public domain in the U.S.A.
Antibody-mediated cell labeling of peripheral T cells with micron-sized iron oxide particles (MPIOs) allows single cell detection by MRI†
Article first published online: 31 MAY 2007
This article is a U.S. Government work and is in the public domain in the U.S.A. Published in 2007 by John Wiley & Sons, Ltd.
Contrast Media & Molecular Imaging
Volume 2, Issue 3, pages 147–153, May/June 2007
How to Cite
Shapiro, E. M., Medford-Davis, L. N., Fahmy, T. M., Dunbar, C. E. and Koretsky, A. P. (2007), Antibody-mediated cell labeling of peripheral T cells with micron-sized iron oxide particles (MPIOs) allows single cell detection by MRI. Contrast Media Mol Imaging, 2: 147–153. doi: 10.1002/cmmi.134
- Issue published online: 2 JUL 2007
- Article first published online: 31 MAY 2007
- Manuscript Accepted: 4 APR 2007
- Manuscript Revised: 29 MAR 2007
- Manuscript Received: 2 FEB 2007
- iron oxide;
- T cells;
- contrast agents
Labeling cells with iron oxide is a useful tool for MRI based cellular imaging. Here it is demonstrated that peripheral rat T cells can be labeled in whole blood, in vitro, with streptavidin-coated micron-sized iron oxide particles (MPIOs), achieving iron concentrations as high as 60 pg iron per cell. This is 30 times the amount of labeling reported with ultrasmall particles of iron oxide (USPIOs). Labeling was mediated by use of a biotinylated anti-CD5 antibody, which is specific for peripheral T cells. Such labeling allowed the in vitro detection of single lymphocytes by MRI, using conditions well suited for in vivo animal work. Electron microscopic analysis demonstrated that MPIOs remained largely extracellular after labeling, with some evidence of intracellular uptake. Cell viability and early and late cytokine release studies showed no significant differences between labeled and unlabeled cells. Therefore, the use of MPIOs for achieving high iron concentrations for cellular MRI is potentially an effective new modality for non-invasive imaging of lymphocytes. Published in 2007 by John Wiley & Sons, Ltd.