Short Communication

Quantitative imaging of the tissue contrast agent [Gd(DTPA)]2− in articular cartilage by laser ablation inductively coupled plasma mass spectrometry

  1. Alessandra Sussulini1,†,‡,
  2. Edzard Wiener2,‡,
  3. Tim Marnitz2,
  4. Bei Wu1,
  5. Berit Müller3,
  6. Bernd Hamm2,
  7. J. Sabine Becker1,*

Article first published online: 11 DEC 2012

DOI: 10.1002/cmmi.1509

Issue

Contrast Media & Molecular Imaging

Contrast Media & Molecular Imaging

Volume 8, Issue 2, pages 204–209, March/April 2013

Additional Information

How to Cite

Sussulini, A., Wiener, E., Marnitz, T., Wu, B., Müller, B., Hamm, B. and Sabine Becker, J. (2013), Quantitative imaging of the tissue contrast agent [Gd(DTPA)]2− in articular cartilage by laser ablation inductively coupled plasma mass spectrometry. Contrast Media Mol Imaging, 8: 204–209. doi: 10.1002/cmmi.1509

Author Information

  1. 1

    Central Division of Analytical Chemistry, Forschungszentrum Jülich, Jülich, Germany

  2. 2

    Charité – Universitätsmedizin Berlin, Department of Radiology, Berlin, Germany

  3. 3

    Charité – Universitätsmedizin Berlin, Department of Pathology, Berlin, Germany

  1. †

    Pontifical Catholic University of Rio de Janeiro (PUC-Rio), Department of Chemistry, Rio de Janeiro, RJ, Brazil

  1. ‡

    The first two authors contributed equally.

*Becker, J. Sabine, Central Division of Analytical Chemistry, Forschungszentrum Jülich, D-52425, Jülich, Germany. E-mail: s.becker@fz-juelich.de

  • ‡

    The first two authors contributed equally.

Publication History

  1. Issue published online: 21 NOV 2012
  2. Article first published online: 11 DEC 2012
  3. Manuscript Accepted: 29 AUG 2012
  4. Manuscript Revised: 21 AUG 2012
  5. Manuscript Received: 23 APR 2012

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Keywords:

  • MRI contrast agents;
  • gadolinium;
  • articular cartilage;
  • imaging;
  • LA-ICP-MS;
  • quantification

Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is an emerging analytical technique in the generation of quantitative images of MR contrast agent distribution in thin tissue sections of articular cartilage. An analytical protocol is described that includes sample preparation by cryo-cutting of tissue sections, mass spectrometric measurements by LA-ICP-MS and quantification of gadolinium images by one-point calibration, standard addition method (employing matrix-matched laboratory standards) and isotope dilution analysis using highly enriched stable Gd-155 isotope (abundance 92 vs 14.8% in the [Gd(DTPA)]2− contrast agent). The tissue contrast agent concentrations of [Gd(DTPA)]2− in cartilage measured in this work are in agreement with findings obtained by magnetic resonance imaging and other analytical methodologies. The LA-ICP-MS imaging data also confirm the observation that the spatial distribution of [Gd(DTPA)]2− in the near-equilibrium state is highly inhomogeneous across cartilage thickness with the highest concentration measured in superficial cartilage and a strong decrease toward the subchondral bone. In the present work, it is shown for the first time that LA-ICP-MS can be applied to validate the results from quantitative gadolinium-enhanced MRI technique of articular cartilage. Copyright © 2012 John Wiley & Sons, Ltd.

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