Labeling of multiple cell lines using a new iron oxide agent for cell tracking by MRI
Article first published online: 29 NOV 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Contrast Media & Molecular Imaging
Volume 6, Issue 6, pages 514–522, November/December 2011
How to Cite
McFadden, C., Mallett, C. L. and Foster, P. J. (2011), Labeling of multiple cell lines using a new iron oxide agent for cell tracking by MRI. Contrast Media Mol Imaging, 6: 514–522. doi: 10.1002/cmmi.456
- Issue published online: 29 NOV 2011
- Article first published online: 29 NOV 2011
- Manuscript Accepted: 16 MAY 2011
- Manuscript Revised: 4 MAY 2011
- Manuscript Received: 17 DEC 2010
- iron oxide;
Stem cells, cancer cells and immune cells were labeled by co-incubation with a new ultra-small iron oxide nanoparticle called Molday ION Rhodamine-B (MIRB). Iron staining, fluorescence imaging, transmission electron microscopy and flow cytometry were used to assess cell viability, function and labeling efficiency. This study has shown that MIRB can be used to label both adherent and nonadherent cell lines, with high viability and loading levels sufficient for their detection in vivo by MRI at 3 T. Copyright © 2011 John Wiley & Sons, Ltd.