Reporter gene imaging using radiographic contrast from nonradioactive iodide sequestered by the sodium–iodide symporter (pages 240–247)
Stephen L. Brown, Svend O. Freytag, Kenneth N. Barton, Michael J. Flynn, Donald J. Peck, Aleksandar F. Dragovic, Ryan Jin, Yener N. Yeni, David P. Fyhrie, Clifford M. Les, Guopei Zhu, Andrew Kolozsvary, Wayne C. Pitchford, S. David Nathanson, Joseph D. Fenstermacher and Jae Ho Kim
Version of Record online: 3 DEC 2007 | DOI: 10.1002/cmmi.150
X-ray attenuation contrast was demonstrated in human tumors grown in mice given systemic iodide and transduced to express the sodium–iodide symporter, NIS, a molecular pump known to sequester iodide. Radiographic contrast was observed using radiographs, conventional X-rays and micro-CT. Clinical CT did not demonstrate convincing contrast in a canine given iodide systemically and whose prostate was transduced with NIS. NIS-sequestered non-radioactive iodide has utility as a reporter gene in small animals, but its clinical applications are questionable.