Contrast Media & Molecular Imaging

Cover image for Contrast Media & Molecular Imaging

May/June 2008

Volume 3, Issue 3

Pages 95–134

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    1. Manganese cell labeling of murine hepatocytes using manganese(III)-transferrin (pages 95–105)

      Christopher H. Sotak, Kathryn Sharer and Alan P. Koretsky

      Article first published online: 10 JUN 2008 | DOI: 10.1002/cmmi.235

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      Manganese(III)-transferrin (Mn(III)-Tf) was investigated as an MRI contrast agent for manganese-labeling of murine hepatocytes via recepto-mediated endocytosis (RME). The degree of manganese cell labeling following incubation for 2--7 h in 31.5 µM Mn(III)- Tf was comparable to that of hepatocytes incubated in 500 µM Mn2+ for 1h. The intrinsic manganese tissue relaxivity between Mn(III)-Tf-labeled and Mn2+-labeled cells was the same. Cellular uptake of Mn2+ via RME involves more than receptor binding alone, conferring more specificity to the entire labeling process.T1-weighted MR images (TR/RE =500/10.4ms) and calculated T1 maps of Mn2+-treated (500µM for 1h) and Mn(III)-Tf-treated (31.5µM for 3h) murine hepatocytes.

    2. Positive MRI contrast enhancement in THP-1 cells with Gd2O3 nanoparticles (pages 106–111)

      Anna Klasson, Maria Ahrén, Eva Hellqvist, Fredrik Söderlind, Anders Rosén, Per-Olov Käll, Kajsa Uvdal and Maria Engström

      Article first published online: 10 JUN 2008 | DOI: 10.1002/cmmi.236

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      Relaxivity measurements of phagocytic THP-1 cells labeled with gadolinium oxide nanoparticles, Gd2O3, showed an r1 of 4.1 and r2 of 17.4 s−1 mm−1. Corresponding results for particles in RPMI 1640 cell culture medium were 3.6 and 12.9 s−1 mm−1. Gd2O3 produced prominent positive contrast when internalized in cells with maximum intensity in samples with a concentration of 0.56 mm Gd2O3.

      Image of two sets of THP-1 cell samples with increasing concentrations of gadolinium from left to right. Incubation concentrations ranged from 0.5 to 2.5 mm (TR = 1000 ms, TE = 20 ms). Signal intensity is high in samples with a final concentration of 0.56 mm Gd2O3 (top row, middle sample, incubation concentration 1.5 mm).

    3. MR imaging assessment of the kinetics of P846, a new gadolinium-based MR contrast medium, in ischemically injured myocardium (pages 112–119)

      Alexis Jacquier, Michael Wendland, Loi Do, Philippe Robert, Claire Corot, Charles B. Higgins and Maythem Saeed

      Article first published online: 11 JUN 2008 | DOI: 10.1002/cmmi.237

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      The objectives of the study were: (1) to compare the kinetics of a new gadolinium-based low-diffusibility MR contrast medium, P846, and Gd-DOTA in left ventricular blood and in normal and ischemically injured myocardium using inversion recovery echo-planar imaging (IR-EPI) and (2) to compare the enhancement pattern after injection of P846 with Gd-DOTA, using T1-weighted spin-echo imaging. Because of its high relaxivity and limited extravasation, P846 can be used with a lower dose of gadolinium in comparison with extracellular gadolinium-based contrast agent.

    4. Kidney volume quantification using contrast-enhanced in vivo X-ray micro-CT in mice (pages 120–126)

      M. Almajdub, L. Magnier, L. Juillard and M. Janier

      Article first published online: 11 JUL 2008 | DOI: 10.1002/cmmi.238

      Thumbnail image of graphical abstract

      Validation of contrast-enhanced micro-CT for in vivo mouse kidney volume measurement is reported, as well as normal values in four strains (BALB/c, C3H/HeN, 129/Sv and C57BL/6J) of healthy male and female mice. The technique provides a useful follow-up research tool for mouse phenotyping and renal disease studies.

  2. Current Awareness

    1. Top of page
    2. Full Papers
    3. Current Awareness
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