Positive MRI contrast enhancement in THP-1 cells with Gd2O3 nanoparticles (pages 106–111)
Anna Klasson, Maria Ahrén, Eva Hellqvist, Fredrik Söderlind, Anders Rosén, Per-Olov Käll, Kajsa Uvdal and Maria Engström
Article first published online: 10 JUN 2008 | DOI: 10.1002/cmmi.236
Relaxivity measurements of phagocytic THP-1 cells labeled with gadolinium oxide nanoparticles, Gd2O3, showed an r1 of 4.1 and r2 of 17.4 s−1 mm−1. Corresponding results for particles in RPMI 1640 cell culture medium were 3.6 and 12.9 s−1 mm−1. Gd2O3 produced prominent positive contrast when internalized in cells with maximum intensity in samples with a concentration of 0.56 mm Gd2O3.
Image of two sets of THP-1 cell samples with increasing concentrations of gadolinium from left to right. Incubation concentrations ranged from 0.5 to 2.5 mm (TR = 1000 ms, TE = 20 ms). Signal intensity is high in samples with a final concentration of 0.56 mm Gd2O3 (top row, middle sample, incubation concentration 1.5 mm).