Internalization of annexin A5-functionalized iron oxide particles by apoptotic Jurkat cells (pages 24–32)
Geralda A. F. van Tilborg, Tessa Geelen, Hans Duimel, Paul H. H. Bomans, Peter M. Frederik, Honorius M. H. F. Sanders, Niko M. Deckers, Roel Deckers, Chris P. M. Reutelingsperger, Gustav J. Strijkers and Klaas Nicolay
Article first published online: 9 JAN 2009 | DOI: 10.1002/cmmi.261
Annexin A5-functionalized iron oxide particles can be used for the detection of apoptosis with MRI. The present study showed that co-incubation of Jurkat cells with the apoptotic stimulus and the annexin A5 nanoparticles leads to membrane-association and internalization of the contrast agent. In contrast, only membrane-associated annexin A5 nanoparticles were observed when incubation was performed after induction of apoptosis. Only little differences were observed between the ΔR2ΔR2 and ΔR2ΔR1 ratios of cell pellets with membrane-associated or internalized annexin A5 nanoparticles.