Serine-derivatized gadonanotubes as magnetic nanoprobes for intracellular labeling (pages 34–38)
Amy A. Hassan, Bonita Tak-Yee Chan, Lesa A. Tran, Keith B. Hartman, Jeyarama S. Ananta, Yuri Mackeyev, Lingyun Hu, Robia G. Pautler, Lon J. Wilson and Adrian V. Lee
Article first published online: 26 JAN 2010 | DOI: 10.1002/cmmi.293
Gadonanotubes (GNTs), which are powerful new T1-weighted MRI contrast agents, were derivatized with serine amino acid substituents to produce water-soluble (2 mg/ml) ser-gadonanotubes (ser-GNTs) as magnetic nanoprobes for intracellular labeling. The ser-GNTs were used to efficiently label MCF-7 human breast cancer cells (1.5 × 109 Gd3+ions/cell) with no observable cytotoxicity. Cell pellets derived from the ser-GNT labeled cells give bright T1-weighted MR images, confirming that the ser-GNTs are a promising new nanoprobe technology for magnetic cell labeling and possibly for in vivo cellular trafficking.