Novel MRI and fluorescent probes responsive to the Factor XIII transglutaminase activity (pages 213–222)
Lorenzo Tei, Galit Mazooz, Yael Shellef, Reut Avni, Katrien Vandoorne, Alessandro Barge, Vyacheslav Kalchenko, Mark W. Dewhirst, Linda Chaabane, Luigi Miragoli, Dario Longo, Michal Neeman and Silvio Aime
Version of Record online: 28 MAY 2010 | DOI: 10.1002/cmmi.392
A novel substrate responsive to transglutaminase optimized by molecular modeling calculations (DCCP16) was coupled both to a Gd–DOTA-like complex and to a fluorophore with the aim at providing molecular imaging of enzymatic activity by MRI and by near-infrared imaging. Results show covalent binding of these contrast agents to human clots, to basement membrane components with a prolonged retention in clots and tumors demonstrating the ability to detect both factor XIII and tissue transglutaminase activities.