Quantitative 1H MRI, 19F MRI, and 19F MRS of cell-internalized perfluorocarbon paramagnetic nanoparticles (pages 19–27)
Maarten B. Kok, Anke de Vries, Desiree Abdurrachim, Jeanine J. Prompers, Holger Grüll, Klaas Nicolay and Gustav J. Strijkers
Article first published online: 21 JUL 2010 | DOI: 10.1002/cmmi.398
In vivo molecular imaging with targeted MRI contrast agents will require sensitive methods to quantify local concentrations of contrast agent. A targeted paramagnetic perfluorocarbon emulsion permits high-resolution 1H MRI combined with quantitative 19F MRI or MRS. In this study we performed in vitro investigations to test whether 1H and 19F MR signals are linear with concentration and can be quantified from the signal in case the emulsion nanoparticles are shuttled into an intracellular compartment by targeting the ανβ3-integrin cell-internalizing receptor.