Hyla Allouche-Arnon, Ayelet Gamliel, Claudia. M. Barzilay, Ruppen Nalbandian, J. Moshe Gomori, Magnus Karlsson, Mathilde H. Lerche and Rachel Katz-Brull
A new hyperpolarized molecular probe, [1,1,2,2-D4,2-13C]choline chloride, enables monitoring of the enzymatic conversion of choline to acetylcholine. The 13C position showed a long T1 of 35 s and a sufficient chemical shift resolution of 2.9 ppm. Following 1.5 h of dynamic nuclear polarization a 24 % polarization has been achieved in liquid state. The T1 of the newly formed hyperpolarized [1,1,2,2-D4,2-13C]acetylcholine was 34 s. We find this new molecular probe to be promising for hyperpolarized metabolic studies.