Fluorescent magnetoliposomes as a platform technology for functional and molecular MR and optical imaging (pages 59–67)
Michael Hodenius, Christian Würth, Jabadurai Jayapaul, John E. Wong, Twan Lammers, Jessica Gätjens, Susanne Arns, Natascha Mertens, Ioana Slabu, Gergana Ivanova, Jörg Bornemann, Marcel De Cuyper, Ute Resch-Genger and Fabian Kiessling
Article first published online: 17 FEB 2012 | DOI: 10.1002/cmmi.467
The fluorophore-normalized fluorescence, quantum yields and fluorescence lifetimes of rhodamine B-containing fluorescent magnetoliposomes (FLU-ML) were substantially reduced by inner filter effects as the magnetoliposome concentration was increased, by increasing molar rhodamine B fraction, and by quenching originating from the iron oxide cores. However, the internalized FLU-ML in human prostata carcinoma cells were clearly visible by fluorescence microscopy. At the FLU-ML concentrations used (up to 3 × 10−3 M Fe), cell viability was not substantially impaired.