Detection of vascular cell adhesion molecule-1 expression with USPIO-enhanced molecular MRI in a mouse model of cerebral ischemia (pages 157–164)
M. Fréchou, V. Beray-Berthat, J.-S. Raynaud, S. Mériaux, F. Gombert, E. Lancelot, M. Plotkine, C. Marchand-Leroux, S. Ballet, P. Robert, G. Louin and I. Margaill
Article first published online: 21 NOV 2012 | DOI: 10.1002/cmmi.1512
There is a clear clinical need for tools to evaluate vascular damage in stroke patients. In this context, P03011, an USPIO (ultrasmall superparamagnetic iron oxide) conjugated with a peptide targeting VCAM-1 (vascular cell adhesion molecule-1) was injected in mice with cerebral ischemia. Brain MRI T2* showed hypointense signals in P03011-treated animals and immunochemistry demonstrated the co-localization of the USPIO with its target. This study provides proof of concept for peptide-conjugated USPIO as tools for in vivo VCAM-1 detection by MRI.