EPR assessment of protein sites for incorporation of Gd(III) MRI contrast labels (pages 252–264)
Jens O. Lagerstedt, Jitka Petrlova, Silvia Hilt, Antonin Marek, Youngran Chung, Renuka Sriram, Madhu S. Budamagunta, Jean F. Desreux, David Thonon, Thomas Jue, Alex I. Smirnov and John C. Voss
Version of Record online: 4 FEB 2013 | DOI: 10.1002/cmmi.1518
The study demonstrates the excellent utility of EPR technique for screening protein candidates as vehicles for carrying MRI contrast agents. A scaled EPR parameter is shown to correlate with the degree of water proton relaxivity for biomolecular MRI contrast agents. Protein contrast agents can thus be screened by X-band EPR spectroscopy employing both the nitroxide and Gd(III)-DOTA probes at sample volumes in the microliter range.