Antibody-functionalized nanoparticles for imaging cancer: influence of conjugation to gold nanoparticles on the biodistribution of 89Zr-labeled cetuximab in mice (pages 402–408)
Linda Karmani, Daniel Labar, Vanessa Valembois, Virginie Bouchat, Praveen Ganesh Nagaswaran, Anne Bol, Jacques Gillart, Philippe Levêque, Caroline Bouzin, Davide Bonifazi, Carine Michiels, Olivier Feron, Vincent Grégoire, Stéphane Lucas, Thierry Vander Borght and Bernard Gallez
Version of Record online: 6 JUN 2013 | DOI: 10.1002/cmmi.1539
Two tracers 89Zr-labeled cetuximab (89Zr-Df-Bz-NCS-cetuximab) and 89Zr-cetuximab conjugated to gold nanoparticles (AuNPs-PPAA-cetuximab-89Zr) were injected in A431 xenograft-bearing mice and their biodistribution was studied using quantitative PET imaging. Radioimmunoconjugates were carefully characterized before injection. Immuno-PET studies revealed no significant difference in tumor uptake for AuNPs-PPAA-cetuximab-89Zr up to 72 h after injection, compared with 89Zr-Df-Bz-NCS-cetuximab and showed a maintaining of high tumor contrast. However, the liver uptake of AuNPs-PPAA-cetuximab-89Zr was higher. In vivo blocking experiments demonstrated selective tumor targeting after coupling reaction.