In vivo visualization of single native pancreatic islets in the mouse (pages 495–504)
Dávid Z. Balla, Sven Gottschalk, G. Shajan, Sandra Ueberberg, Stephan Schneider, Matthias Hardtke-Wolenski, Elmar Jaeckel, Verena Hoerr, Cornelius Faber, Klaus Scheffler, Rolf Pohmann and Jörn Engelmann
Article first published online: 25 DEC 2013 | DOI: 10.1002/cmmi.1580
Direct visualization of single native pancreatic islets in vivo after i.v. administration of a targeted contrast agent was demonstrated in the mouse by performing MRI at 16.4 T. The necessary signal efficiency and contrast was achieved using ferromagnetic cobalt nanoparticles as magnetically active agents functionalized with the single-chain antibody fragment SCA B1, and by the optimization of the MR-acquisition and reconstruction protocols. Results were confirmed by ex vivo MRI, immunohistochemistry and numerical simulations.