Contrast Media & Molecular Imaging

Cover image for Vol. 9 Issue 2

March/April 2014

Volume 9, Issue 2

Pages i–iii, 109–186

  1. Issue Information

    1. Top of page
    2. Issue Information
    3. Full Papers
    4. Short Communications
    1. Issue Information (pages i–iii)

      Version of Record online: 12 FEB 2014 | DOI: 10.1002/cmmi.1593

  2. Full Papers

    1. Top of page
    2. Issue Information
    3. Full Papers
    4. Short Communications
    1. Iron-loaded PLLA nanoparticles as highly efficient intracellular markers for visualization of mesenchymal stromal cells by MRI (pages 109–121)

      I. Vernikouskaya, N. Fekete, M. Bannwarth, A. Erle, M. Rojewski, K. Landfester, G. Schmidtke-Schrezenmeier, H. Schrezenmeier and V. Rasche

      Version of Record online: 12 FEB 2014 | DOI: 10.1002/cmmi.1544

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      High R2* relaxivity rate of nanometer-sized iPLLA enables visualization of a single labelled cell in vitro and allows sensitive in vivo and detection of local cell aggregations at a relatively low payload of MR contrast.

    2. A fast tumor-targeting near-infrared fluorescent probe based on bombesin analog for in vivo tumor imaging (pages 122–134)

      Haiyan Chen, Shunan Wan, Fenxia Zhu, Chuan Wang, Sisi Cui, Changli Du, Yuxiang Ma and Yueqing Gu

      Version of Record online: 12 FEB 2014 | DOI: 10.1002/cmmi.1545

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      PEG and bombesin analog modified near-infrared fluorescence probe exhibited fast and high tumor targeting capability, which could act as an effective fluorescence probe for tumor diagnosis.

    3. In vivo near-infrared fluorescence targeting of T cells: comparison of nanobodies and conventional monoclonal antibodies (pages 135–142)

      Peter Bannas, Lennart Well, Alexander Lenz, Björn Rissiek, Friedrich Haag, Joanna Schmid, Katja Hochgräfe, Martin Trepel, Gerhard Adam, Harald Ittrich and Friedrich Koch-Nolte

      Version of Record online: 12 FEB 2014 | DOI: 10.1002/cmmi.1548

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      We assessed the utility of nanobodies (17 kDa) and nanobody-Fc fusion proteins (82 kDa) as alternatives to monoclonal antibodies (150 kDa) as theranostics, using T cell ADP-ribosyltransferase 2 (ART2) as model antigen for specific targeting of lymph nodes. Our results revealed, that small single domain nanobodies allow not only in vivo imaging of lymph nodes with excellent target-to-background ratio, but also short term inhibition of specific T cell ecto-enzymes in vivo. Larger nanobody-FC-fusion proteins are less suited for in vivo imaging due to higher unspecific background signals, but enable long-term inhibition of T cell ecto-enzymes in vivo.

    4. Multimodal cell tracking of a spontaneous metastasis model: comparison between MRI, electron paramagnetic resonance and bioluminescence (pages 143–153)

      Pierre Danhier, Géraldine De Preter, Julie Magat, Quentin Godechal, Paolo E. Porporato, Bénédicte F. Jordan, Olivier Feron, Pierre Sonveaux and Bernard Gallez

      Version of Record online: 12 FEB 2014 | DOI: 10.1002/cmmi.1553

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      Ex vivo electron paramagnetic resonance allows short-term cell tracking of iron oxide-labeled metastatic cancer cells whereas bioluminescence imaging allows long-term monitoring of cells. Magnetic resonance imaging is suitable labeled cells to be checked (hypointense spot on T2-, T2*-weighted images) at their injection site.

    5. Influence of paramagnetic melanin on the MRI contrast in melanoma: a combined high-field (11.7 T) MRI and EPR study (pages 154–160)

      Q. Godechal, L. Mignion, O. Karroum, J. Magat, P. Danhier, R. Morandini, G. E. Ghanem, P. Leveque and B. Gallez

      Version of Record online: 12 FEB 2014 | DOI: 10.1002/cmmi.1554

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      The origin of the MRI contrast pattern observable in melanoma remains a source of controversy. T1, T2 and T2* contrast was analyzed at 11.7 T using four types of experimental tumors with different pigmentations, and the paramagnetic content in melanin pigment was measured by EPR. No significant correlation was observed between the content in melanin and the relaxation times T1, T2 and T2*, emphasizing that the presence of pigment alone has negligible effect on the MRI contrast.

    6. Anatomical and functional imaging of myocardial infarction in mice using micro-CT and eXIA 160 contrast agent (pages 161–168)

      Jeffrey R. Ashton, Nicholas Befera, Darin Clark, Yi Qi, Lan Mao, Howard A. Rockman, G. Allan Johnson and Cristian T. Badea

      Version of Record online: 12 FEB 2014 | DOI: 10.1002/cmmi.1557

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      eXIA 160 is a blood-pool contrast agent for micro-CT which shows uptake in metabolically active tissues, including the myocardium and brown adipose tissue. Infarcted myocardium shows no uptake of contrast agent, making it easy to differentiate from non-infarcted myocardium. Both cardiac function and infarct size can be succesfully measured using micro-CT with results comparable to SPECT imaging but at higher spatial resolution.

    7. In vitro Gd-DTPA relaxometry studies in oxygenated venous human blood and aqueous solution at 3 and 7 T (pages 169–176)

      Chaitanya Kalavagunta, Shalom Michaeli and Gregory J. Metzger

      Version of Record online: 12 FEB 2014 | DOI: 10.1002/cmmi.1568

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      In vitro T1 and T2* relaxivities (r1 and r2*) of Gd-DTPA (GaD) in oxygenated human venous blood (OVB) and aqueous solution (AS) at 3 and 7 T were calculated at 37 ± 2 °C. A linear relationship was observed between [GaD] and R1 in AS and OVB at 3 and 7 T. A linear relationship for R2* was observed in AS. In OVB, R2* had a quadratic relationship with [GaD] below 2 mm and a linear relationship above 2 mm at both field strengths.

  3. Short Communications

    1. Top of page
    2. Issue Information
    3. Full Papers
    4. Short Communications
    1. Sensitivity-enhanced chemical exchange saturation transfer (CEST) MRI with least squares optimization of Carr Purcell Meiboom Gill multi-echo echo planar imaging (pages 177–181)

      Phillip Zhe Sun, Yu Wang and Jie Lu

      Version of Record online: 12 FEB 2014 | DOI: 10.1002/cmmi.1546

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      The magnitude of CEST effect for dilute CEST agents undergoing slow or intermediate chemical exchange is typically small, requiring the use of signal averaging to enhance its sensitivity. Given that T2-induced signal loss can be normalized by asymmetry analysis, CEST multi-echo (CESTme) echo planar imaging can be superimposed to enhance CEST MRI sensitivity. Using least-squares optimization, the sensitivity of CESTme MRI was significantly higher than that obtained by conventional single echo acquisition, demonstrating a sensitivity-enhanced CEST MRI technique.

    2. You have full text access to this OnlineOpen article
      Quantitation of a spin polarization-induced nuclear Overhauser effect (SPINOE) between a hyperpolarized 13C-labeled cell metabolite and water protons (pages 182–186)

      Irene Marco-Rius, Sarah E. Bohndiek, Mikko I. Kettunen, Timothy J. Larkin, Meer Basharat, Colm Seeley and Kevin M. Brindle

      Version of Record online: 12 FEB 2014 | DOI: 10.1002/cmmi.1556

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      A theoretical expression for the expected SPINOE enhancement of solvent protons in an aqueous solution of hyperpolarized [1,413C2]fumarate and its experimental validation are presented.

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