Original Article

You have full text access to this OnlineOpen article

Loss of E-cadherin expression resulting from promoter hypermethylation in oral tongue carcinoma and its prognostic significance

  1. Hsiao Wen Chang Ph.D.1,
  2. Vivian Chow M.Phil.1,
  3. King Yin Lam FRACP2,
  4. William Ignace Wei M.S.1,
  5. Anthony Po Wing Yuen M.S.1,†,*

Article first published online: 18 JAN 2002

DOI: 10.1002/cncr.10211

Issue

Cancer

Cancer

Volume 94, Issue 2, pages 386–392, 15 January 2002

Additional Information

How to Cite

Chang, H. W., Chow, V., Lam, K. Y., Wei, W. I. and Wing Yuen, A. P. (2002), Loss of E-cadherin expression resulting from promoter hypermethylation in oral tongue carcinoma and its prognostic significance. Cancer, 94: 386–392. doi: 10.1002/cncr.10211

Author Information

  1. 1

    Department of Surgery, The University of Hong Kong, Queen Mary Hospital, Hong Kong, SAR China

  2. 2

    Department of Pathology, The University of Hong Kong, Queen Mary Hospital, Hong Kong, SAR China

  1. †

    Fax: (011) 852-2855-3464

*Department of Surgery, The University of Hong Kong, Queen Mary Hospital, Pokfulam Road, Hong Kong, SAR China

Publication History

  1. Issue published online: 18 JAN 2002
  2. Article first published online: 18 JAN 2002
  3. Manuscript Accepted: 24 SEP 2001
  4. Manuscript Revised: 29 AUG 2001
  5. Manuscript Received: 21 MAY 2001

Funded by

  • Kadoorie Cancer Research Fund
  • The University of Hong Kong

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (471K)

REFERENCES

  • 1
    Yuen APW, Wei WI, Wong YM, Tang KC. Elective neck dissection versus observation in the surgical treatment of early oral tongue carcinoma. Head Neck 1997; 19: 583-8.
    Direct Link:
  • 2
    Yuen APW, Lam KY, Chan ACL, Wei WI. Clinicopathological analysis of elective neck dissection for N0 neck of early oral tongue carcinoma. Am J Surg 1999; 177: 90-2.
  • 3
    Yuen PW, Wei WI, Wong YM, Tang KC. Comprehensive analysis of results of surgical treatment of oral tongue carcinoma in Hong Kong. Chinese Med J 1997; 110: 859-64.
  • 4
    Yuen APW, Wei WI, Lam LK, Ho WK, Kwong D. Results of surgical salvage of locoregional recurrence of carcinoma of tongue after radiotherapy failure. Ann Otol Rhinol Laryngol 1997; 106: 779-82.
  • 5
    Yuen APW, Lam KY, Chan ACL, Wei WI, Lam LK. Clinicopathological analysis of local spread of carcinoma of tongue. Am J Surg 1998; 175: 242-4.
  • 6
    Yuen APW, Lam KY, Wei WI, Ho WK. A comparison of prognostic significance of tumor diameter, length, width, thickness, area, volume and clinicopathological features of oral tongue carcinoma. Am J Surg 2000; 180: 139-43.
  • 7
    Perl AK, Wilgenbus P, Dahl U, Semb H, Christofori G. A causal role for E-cadherin in the transition from adenoma to carcinoma. Nature 1998; 392: 190-3.
  • 8
    Vleminckx K, Vakaet L Jr., Mareel M, Fiers W, van Roy F. Genetic manipulation of E-cadherin expression by epithelial tumor cells reveals an invasion suppressor role. Cell 1991;66: 107-19.
  • 9
    Mareel M, Bracke M, Van Roy F. Cancer metastasis: negative regulation by an invasion-suppressor complex. Cancer Detect Prev 1995; 19: 451-64.
  • 10
    Behrens J, Lowrick O, Klein-Hitpass L, Birchmeier W. The E-cadherin promoter: functional analysis of a G.C-rich region and an epithelial cell-specific palindromic regulatory element. Proc Natl Acad Sci USA 1991;88: 11495-9.
  • 11
    Hennig G, Lowrick O, Birchmeier W, Behrens J. Mechanisms identified in the transcriptional control of epithelial gene expression. J Biol Chem 1996; 271: 595-602.
  • 12
    Yoshiura K, Kanai Y, Ochiai A, Shimoyama Y, Sugimura T, Hirohashi S. Silencing of the E-cadherin invasion-suppressor gene by CpG methylation in human carcinomas. Proc Natl Acad Sci USA 1995; 92: 7416-9.
  • 13
    Kanai Y, Ushijima S, Hui AM, Ochiai A, Tsuda H, Sakamoto M, et al. The E-cadherin gene is silenced by CpG methylation in human hepatocellular carcinomas. Int J Cancer 1997; 71: 355-9.
    Direct Link:
  • 14
    Graff JR, Herman JG, Lapidus RG, Chopra H, Xu R, Jarrard DF, et al. E-cadherin expression is silenced by DNA hypermethylation in human breast and prostate carcinomas. Cancer Res 1995; 55: 5195-9.
  • 15
    Gray SG, Eriksson T, Ekstrom TJ. Methylation, gene expression and the chromatin connection in cancer. Int J Mol Med 1999; 4: 333-50.
  • 16
    Ohtani-Fujita N, Fujita T, Aoike A, Osifchin NE, Robbins PD, Sakai T. CpG methylation inactivates the promoter activity of the human retinoblastoma tumor-suppressor gene. Oncogene 1993; 8: 1063-7.
  • 17
    Chow V, Yuen APW, Lam KY, Ho WK, Wei WI. A comparative study of the clinicopathological significance of E-cadherin and catenins (α,β,γ) expression in the surgical management of oral tongue carcinoma. J Cancer Res Clin Oncol 2001; 127: 59-63.
  • 18
    Chang HW, Chong SM, Peh SC, Lee SH. X-chromosome inactivation analysis of isolated Reed-Sternberg cells in nodular sclerosing Hodgkin's disease. Br J Haematol 1999; 107: 641-7.
    Direct Link:
  • 19
    Issa JP, Ottaviano YL, Celano P, Hamilton SR, Davidson NE, Baylin SB. Methylation of the oestrogen receptor CpG island links ageing and neoplasia in human colon. Nat Genet 1994; 7: 536-40.
  • 20
    Singer-Sam J, Grant M, LeBon JM, Okuyama K, Chapman V, Monk M, et al. Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation. Mol Cell Biol 1990; 10: 4987-9.
  • 21
    Frommer M, McDonald LE, Millar DS, Collis CM, Watt F, Grigg GW, et al. A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands. Proc Natl Acad Sci USA 1992; 89: 1827-31.
  • 22
    Herman JG, Graff JR, Myohanen S, Nelkin BD, Baylin SB. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci USA 1996; 93: 9821-6.
  • 23
    Hayatsu H, Miller RC. The cleavage of DNA by the oxygen-dependent reaction of bisulfite. Biochem Biophys Res Commun 1972; 46: 120-4.
  • 24
    Nass SJ, Herman JG, Gabrielson E, Iversen PW, Parl FF, Davidson NE, et al. Aberrant methylation of the estrogen receptor and E-cadherin 5' CpG islands increases with malignant progression in human breast cancer. Cancer Res 2000; 60: 4346-8.
  • 25
    Graff JR, Greenberg VE, Herman JG, Westra WH, Boghaert ER, Ain KB, et al. Distinct patterns of E-cadherin CpG island methylation in papillary, follicular, Hurthle's cell, and poorly differentiated human thyroid carcinoma. Cancer Res 1998; 58: 2063-6.
  • 26
    Kanai Y, Ushijima S, Hui AM, Ochiai A, Tsuda H, Sakamoto M, et al. The E-cadherin gene is silenced by CpG methylation in human hepatocellular carcinomas. Int J Cancer 1997; 71: 355-9.
    Direct Link:
  • 27
    Behrens J, Lowrick O, Klein-Hitpass L, Birchmeier W. The E-cadherin promoter: functional analysis of a G.C-rich region and an epithelial cell-specific palindromic regulatory element. Proc Natl Acad Sci USA 1991;88: 11495-9.
  • 28
    Ji X, Woodard AS, Rimm DL, Fearon ER Transcriptional defects underlie loss of E-cadherin expression in breast cancer. Cell Growth Differ 1997; 8: 773–8.
  • 29
    Chan MF, Liang G, Jones PA. Relationship between transcription and DNA methylation. Curr Top Microbiol Immunol 2000; 249: 75–86.
  • 30
    Kass SU, Pruss D, Wolffe AP How does DNA methylation repress transcription? Trends Genet 1997; 13: 444–9.
  • 31
    Mancini DN, Singh SM, Archer TK, Rodenhiser DI. Site-specific DNA methylation in the neurofibromatosis (NF1) promoter interferes with binding of CREB and SP1 transcription factors. Oncogene 1999; 18: 4108-9.
  • 32
    Harrington MA, Jones PA, Imagawa M, Karin M. Cytosine methylation dose not affect binding of transcription factor Sp1. Proc Natl Acad Sci USA 1998; 85: 2066–70.
  • 33
    Holler M, Westin G, Jiricny J, Schaffner W. Sp1 transcription factor binds DNA and activates transcription even when the binding site is CpG methylated. Genes Dev 1988; 2: 1127-35.
  • 34
    Clark SJ, Harrison J, Molloy PL. Sp1 binding is inhibited by (m)Cp(m)CpG methylation. Gene 1997; 195: 67-71.
  • 35
    Graff JR, Gabrielson E, Fujii H, Baylin SB, Herman JG. Methylation patterns of the E-cadherin 5' CpG island are unstable and reflect the dynamic, heterogeneous loss of E-cadherin expression during metastatic progression. J Biol Chem 2000; 275: 2727-32.
  • 36
    Siitonen SM, Kononen JT, Helin HJ, Rantala IS, Holli KA, Isola JJ. Reduced E-cadherin expression is associated with invasiveness and unfavorable prognosis in breast cancer. Am J Clin Pathol 1996; 105: 394-402.
  • 37
    Tamura G, Yin J, Wang S, Fleisher AS, Zou T, Abraham JM, et al. E-cadherin gene promoter hypermethylation in primary human gastric carcinomas. J Natl Cancer Inst 2000; 92: 569-73.
View Full Article (HTML) Get PDF (471K)