THIS ARTICLE HAS BEEN RETRACTED
Retracted: Radioimmunotherapy of small-volume disease of metastatic colorectal cancer
Results of a phase II trial with the iodine-131–labeled humanized anti–carcinoembryonic antigen antibody hMN-14
Version of Record online: 12 FEB 2002
Copyright © 2002 American Cancer Society
Supplement: Eighth Conference on Radioimmunodetection and Radioimmunotherapy of Cancer
Volume 94, Issue Supplement 4, pages 1373–1381, 15 February 2002
How to Cite
Behr, T. M., Liersch, T., Greiner-Bechert, L., Griesinger, F., Béhé, M., Markus, P. M., Gratz, S., Angerstein, C., Brittinger, G., Becker, H., Goldenberg, D. M. and Becker, W. (2002), Retracted: Radioimmunotherapy of small-volume disease of metastatic colorectal cancer. Cancer, 94: 1373–1381. doi: 10.1002/cncr.10308
- Issue online: 12 FEB 2002
- Version of Record online: 12 FEB 2002
- Manuscript Accepted: 14 NOV 2001
- Manuscript Received: 31 OCT 2001
- Deutsche Forschungsgemeinschaft. Grant Number: Be 1689/4-2
Retraction: Retraction: “Radioimmunotherapy of small-volume disease of metastatic colorectal cancer: Results of a phase II trial with the iodine-131–labeled humanized anti–carcinoembryonic antigen antibody hMN-14,”
Vol. 121, Issue 13, 2290, Version of Record online: 30 MAR 2015
- colorectal cancer;
- carcinoembryonic antigen;
- humanized anti–carcinoembryonic antigen antibody;
- small-volume metastatic disease;
- adjuvant therapy
Whereas radioimmunotherapy (RIT) has shown disappointing results in bulky, solid tumors, preclinical results in small-volume disease and in an adjuvant setting are promising. In a previous Phase I study, the authors had encouraging results with the iodine-131 (131I)–labeled humanized anti–carcinoembryonic antigen (anti-CEA) antibody (MAb) hMN-14 in small-volume disease of colorectal cancer. The aim of this study was to evaluate, in a subsequent Phase II trial, the therapeutic efficacy of this 131I-labeled humanized anti-CEA antibody in colorectal cancer patients with small-volume disease or in an adjuvant setting.
Thirty colorectal cancer patients, with small-volume metastatic disease (n = 21; all lesions ≤ 3.0 cm, and chemorefractory to 5-fluorouracil and folinic acid) or in an adjuvant setting (n = 9), 4–6 weeks after surgical resection of liver metastases with curative intention, were studied. The patients were given a single injection of 131I-hMN-14 immunoglobulin G at a 60 mCi/m2 dose level, which was shown to be the maximum tolerated dose in the previous Phase I study. Follow-up was obtained at 3-month intervals for as long as 36 months.
At a mean blood-based red marrow dose of 1.8 ± 0.8 Gy, myelotoxicity was the only toxicity observed, but only 1 of 28 assessable patients developed transient Grade 4 thrombocytopenia. Of the 21 patients with radiologically documented lesions, 19 were assessable. Three experienced partial remission and eight showed minor responses up to 15 months in duration (corresponding to an objective response rate of 16% and an overall response rate of 58%; the mean duration of response was 9 months). At the time this article was written, seven of nine patients in the adjuvant setting had remained free of disease for up to 36 months (one patient relapsed after 6 months and another after 30 months), whereas the relapse rate in a historical control group receiving chemotherapy was 67% over the same time period. Five patients with radiologically documented lesions, having experienced at least disease stabilization as a consequence of RIT, were retreated at the same 60-mCi/m2 dose level at 8–16 months after the first therapy. No evidence of increased toxicity was observed (no hematologic toxicity was higher than Grade 3). Two of four assessable retreated patients experienced partial remissions; one of these four again experienced disease stabilization as a consequence of the second radioantibody therapy injection.
These data suggest that RIT is a safe and effective form of therapy for small-volume colorectal cancer and has potential as treatment for colorectal cancer in an adjuvant setting. Toxicity is restricted to mild and transient leuko- and thrombocytopenia. Retreatment seems to be a feasible option. A prospective randomized comparison with standard chemotherapy is indicated. Cancer 2002;94:1373–81. © 2002 American Cancer Society.
Colorectal cancer, which comprises 15% of all malignancies, is one of the most frequently occurring cancer types in both genders.1 To date, surgery is the only potentially curative therapeutic modality.1 However, despite the introduction of adjuvant chemotherapeutic regimens, which may reduce the relapse rate by approximately 30%,2 the disease will still relapse in approximately one-half of patients. In unresectable cases of metastatic disease, the 5-year survival is close to zero,1 despite several promising new chemotherapeutic developments, such as the introduction of the semisynthetic camptothecin derivative, irinotecan as a topoisomerase-I inhibitor, orally administrable fluoropyrimidines, or the clinical use of oxaliplatin as a novel platinum derivative with proven therapeutic efficacy in gastrointestinal cancers.3–5 Even in cases of resectable metastatic disease, approximately two-thirds of patients will die within 5 years of relapsing cancer. Adjuvant chemotherapy has failed to improve survival rates, e.g., for patients after surgical resection of liver metastases performed with curative intent.6
Immunotherapy directed against the 41-kD cell surface glycoprotein EpCAM has shown, in an adjuvant setting with colorectal cancer, results comparable to those obtained with adjuvant chemotherapy,7, 8 which is in contrast to its lack of significant antitumor effects in established metastatic disease.9, 10 On the other hand, immunotherapy has shown promising therapeutic results in breast and hematologic malignancies with established metastases.11, 12
In this context, radioimmunotherapy (RIT) appears to be an attractive therapeutic concept, as it can deliver tumoricidal radiation doses to tumors that may be too large to respond to a purely immunologic approach.10 Indeed, in radiosensitive tumors, such as non-Hodgkin lymphoma, RIT has led to long-term remissions or even cures in a high percentage of patients.13, 14 In solid tumors, however, success is still limited, probably due to the low specific accretion of the radiolabeled antibody in the tumor target as compared with normal tissues.10 However, since the tumor uptake, and thus the radiation dose to the tumor, increase exponentially with decreasing tumor size,15 RIT may be a viable option, especially for small-volume and minimal residual disease.15–17
In a previous communication, we were able to demonstrate, in a model of human colon cancer metastatic to the liver of nude mice, that in small-volume disease, RIT may be superior to conventional chemotherapy with 5-fluorouracil (5-FU) and leucovorin or with irinotecan. Antibodies of higher affinity proved to be clearly superior to those of lower affinity.17 In a subsequently designed clinical Phase I trial using hMN-14, a 131I-labeled, humanized, high-affinity anti–carcinoembryonic antigen (anti-CEA) monoclonal antibody (MAb), the toxicity and therapeutic efficacy of RIT in colorectal cancer patients with small-volume disease metastatic to the liver was determined.17 The maximum tolerated activity/dose (MTD) of 131I-hMN-14 was reached at 60 mCi/m2, transient mild-to-moderate myelotoxicity being dose-limiting. In 11 assessable patients, two had partial remissions (corresponding to an objective response rate of 18%) and five (45%) had minor or mixed responses or experienced stabilization of previously rapidly progressing disease. We concluded that the clinical response rates in patients with small-volume disease were encouraging, comparable to the response rates of conventional chemotherapeutic regimens but with fewer side effects.17 The aim of the current Phase II study was to further evaluate the therapeutic efficacy of 131I-labeled hMN-14 in colorectal cancer patients with small-volume disease or in an adjuvant setting.
Materials and Methods
Antibodies and Radiolabeling
hMN-14 is a high affinity (Ka = 109L/mol) humanized immunoglobulin (Ig)G monoclonal anti-CEA antibody (obtained from Immunomedics, Inc., Morris Plains, NJ).18, 19 Preclinical and clinical experiences with this antibody have been described previously.18, 19 It is directed against a Class III epitope of the CEA molecule, according to the classification of Primus et al.20
Iodine-131 (131I)was purchased as sodium iodide in 0.1 M NaOH from Nordion (Fleurus, Belgium). Radioiodination was performed using the iodogen method, as described previously.17 The antibody, in phosphate-buffered saline, was transferred into an iodogen-coated glass vial (500 μg iodogen coating the inner surface of a 10-mL vial) with a magnetic stir bar placed inside. Sodium phosphate buffer (0.5 M, pH 7.4) was added in a volume twice as great as the volume of the radioiodine to be used. The specific activity used was 15–20 mCi/mg. The vial was placed on a magnetic stirrer, and the activity was added to 2.5 mL sodium phosphate, (0.04 M, pH 7.4). After a stirring time of 10–15 minutes, Dowex 1X8-100 anion exchange resin (Cl− form, Sigma, Deisenhofen, Germany) was added and the incubation time prolonged for another 5 minutes. Subsequently, the radioiodinated antibody was filtered through a sterile Millex-GV filter (pore size, 0.22 μm; Millipore, Molsheim, France). The quality of each preparation was tested by instant thin layer chromatography and size-exclusion high-performance liquid chromatography. The amount of unbound radioiodine was less than 2% in each preparation;17 the immunoreactivity was ≥ 85% in all cases.
Patients and Antibody Administration
Thirty colorectal cancer patients were studied; some had small-volume metastatic disease (n = 21; all lesions ≤ 3.0 cm, and all either chemorefractory to 5-FU and folinic acid or chemointolerant) and some were in an adjuvant setting (n = 9), at 4–6 weeks after surgical resection of liver metastases performed with curative intent. All had histologically proven, CEA-expressing colorectal cancer. The patients were at least 4 weeks beyond major surgery or external beam radiation, and had a performance status of 60 or greater on the Karnofsky scale.
All patients underwent thorough staging procedures within 1 week before RIT, including whole-body (i.e., chest to pelvis) computed tomography (CT), abdominal ultrasonography, bone scan, and, optionally, magnetic resonance tomography of selected regions and 18F-FDG whole-body positron emission tomography. After informed consent had been obtained, all patients were premedicated with 200 mg potassium iodide daily, initiated 24 hours before the antibody administration to decrease thyroid and gastric uptake, respectively.21 This medication was continued until radiation restrictions were lifted for the patients.
The radiolabeled antibody was infused during a 10- to 30-minute period in a volume of 10–30 mL sterile 0.9% NaCl containing 1.0–2.5% human serum albumin. The patients were given single injections at the 60 mCi/m2 dose level established in the previous Phase I trial as the MTD.17 Routine blood chemistry parameters, blood cell counts, and differential blood counts were obtained weekly until 8 weeks posttherapy, and then monthly thereafter.
Imaging and Dosimetry
Scanning was performed with a Picker Prism 2000 dual-head gamma camera, equipped with high-energy collimators (Picker/Marconi, Cleveland, OH). Anterior and posterior whole-body scans were obtained daily from the day when the patients' whole-body activity fell below 40 mCi (i.e., from Day 2–3 postinjection) up to 10–14 days postinjection. MAb blood clearance rates were determined by counting samples at various times after the end of the infusion. Total-body clearance rates were determined from whole-body scans and hand-held rate meter measurements.
For organ and tumor dosimetry, regions of interest (ROIs) of the whole body, organs, and visible tumors were generated from the anterior and posterior planar views. All calculations were performed using PC software developed for this purpose.22, 23 The blood time-activity concentration data were fit by a biexponential function to obtain the cumulated activity in the blood. The red marrow cumulated activity was calculated from the blood data by multiplying this concentration by 1500, as the assumed weight in grams of the marrow in an average adult. Time-activity curves were generated and integrated, and cumulated activities as well as residence times were derived.22 These data were entered into the MIRDOSE3 program,23 which yields the organ, red marrow, tumor, and whole-body dosimetry according to the MIRD scheme, based on derived residence times.
All patients underwent CT of the chest, abdomen, and pelvis on the day before therapy, as well as 4 and 12 weeks later. Afterwards, patients were evaluated in 3-month intervals, including clinical follow-up, sonography of the abdomen, whole-body CT, routine blood chemistry and tumor marker evaluations.
Therapeutic responses were graded as follows, according to oncologic standard criteria:
- 1Complete remission: absence of clinically detectable disease, with complete normalization of serum tumor marker (CEA) levels, for at least 1 month;
- 2Partial remission : at least 50% reduction in the sum of the diameter of measurable disease without appearance of new lesions and without increase in size of any lesion, for at least 1 month;
- 3Minor/mixed response - less than 50% reduction in sum of the diameters of measurable lesions without an increase in the size of any lesion;
- 4Progression : greater than 25% increase in measurable disease and/or any new metastasis during treatment.
Patients' Medical Histories
Twenty-one patients with measurable, radiologically documented small-volume metastatic colorectal cancer were enrolled in this Phase II study of the 131I-labeled high-affinity humanized anti-CEA MAb hMN-14. These patients' characteristics are summarized in Table 1. Nine women and 12 men presented with small-volume metastatic disease (defined as all lesions being less than 3.0 cm in maximum diameter). The vast majority of them were pretreated with chemotherapy (5-FU alone or in combination with leucovorin) and/or external beam radiotherapy (Tables 1 and 2). These conventional treatment regimens had either failed or were abandoned because of severe side effects, such as mucositis and diarrhea. Eleven patients had liver metastases, six had lung metastases (one of them with additional lymph node metastases), three had both liver and lung lesions, and one presented with a peritoneal carcinomatosis. Serum CEA levels were mildly to moderately elevated (range, 3.9–45 ng/mL, data not shown). Sufficient follow-up was available for 19 of these 21 patients; of the other two, one committed suicide shortly after being released from the hospital after RIT, and the other was lost to follow-up.
|Patient IDa||Gender||Site of primary tumor||Previous chemo- and/or radiotherapy||Time from primary diagnosis||Actual metastatic site||Response to RIT||Duration of response (mos)|
|(E1)||M||Colon||5-FU/LV, CPT-11||3 yrs||Liver||PR||12|
|(E2)||M||Rectum||5-FU/LV, pelvic EBRT||2 yrs||Lung||PR||3|
|(E3)||F||Colon||5-FU/LV||4 yrs||Mesenteric root, Peritoneum||PR||15|
|(E4)||F||Rectum||5-FU/LV, pelvic EBRT||4 yrs||Lung||Minor||3|
|(E5)||M||Rectum||5-FU/LV, pelvic EBRT||4 yrs||Lung, lymph nodes||Minor||9|
|(E6)||M||Rectum||pelvic EBRT, CPT-11||5 yrs||Liver, lung||Minor||10|
|(E7)||F||Rectum||5-FU/LV, pelvic EBRT||4 yrs||Lung||Minor||6|
|(E9)||M||Rectum||5-FU/LV, pelvic EBRT||2 yrs||Lung||Minor||11|
|(E10)||M||Rectum||5-FU/LV, pelvic EBRT||2 yrs||Liver, lung||Minor||7|
|(E11)||F||Rectum||pelvic EBRT, 5-FU||1 yr||Liver||Minor||5|
|(E13)||F||Rectum||5-FU/LV, pelvic EBRT||1 yr||Liver||PD||--|
|(E16)||M||Colon||5-FU/LV||1 yr||Liver, lung||PD||--|
|(E20)||F||Colon||5-FU/LV, CPT-11||1 yr||Liver||N/A||N/A|
|Patient IDa||Gender||Site of primary tumor||Previous chemo- and/or radiotherapy||Time to hepatic relapse||Time after liver surgery (weeks)||Disease-free interval (duration of response) (mos)||Site of relapse|
|(A2)||M||Rectum||5-FU/LV, pelvic EBRT||2 yrs||4||30||Liver|
|(A4)||F||Colon||5-FU/LV, CPT-11||4 yrs||4||27+||--|
|(A5)||M||Rectum||Pelvic EBRT||2 yrs||4||27+||--|
|(A7)||M||Rectum||5-FU/LV, pelvic EBRT||7 mos||4||24+||--|
|(A8)||M||Rectum||5-FU/LV, pelvic EBRT||9 mos||6||24+||--|
|(A9)||F||Colon||5-FU/LV, CPT-11||6 yrs||4||6||Liver, lung|
Nine patients in an adjuvant setting, at 4–6 weeks after surgical resection of metachronous liver metastases performed with curative intent, were included. Their characteristics are shown in Table 2. Four were women and five were men. The median time between primary diagnosis and the first appearance of liver metastases was 2 years (range, 3 months to 6 years). Again, serum CEA levels were mildly to moderately elevated in these patients (range, 10.8–42 ng/mL, data not shown).
Side Effects and Toxicity
The therapy infusion was tolerated well by all patients. The only acute side effect observed was occasional mild nausea, frequently associated with a metallic taste, occurring in approximately one-half of the patients, most likely caused by the stomach-irritating effects of the concomitant cold iodine medication intended to block the thyroid from metabolically liberated radioiodide. As expected, based on the results of the previous Phase I study17 and the radiation dosimetry (Table 3), the red marrow was the only dose-limiting organ. All patients were treated at a 60 mCi/m2 dose level, which was identified as the MTD in the previous Phase I study.17 At mean blood-based red marrow doses of 1.8 ± 0.8 Gy, typically white blood cell and platelet counts began to drop, platelets usually preceding leukocytes, 2–3 weeks after RIT, reaching their nadir 4–7 weeks after the RIT injection. The time to recovery was typically 9–11 weeks post-RIT. Most of the time, thrombo- and leukocytopenia were mild to moderate (WHO Grade 2 or 3), and only 1 of 28 assessable patients developed a Grade 4 thrombocytopenia lasting for 10 days and a Grade 3 leukopenia lasting for 7 days. It is noteworthy that this patient was the only one who had previously received therapy containing mitomycin, which is known as potent and long-lasting radiosensitizing chemotherapeutic agent.24 Another patient with biopsy-proven bone marrow involvement with tumor cells (Patient E3; Table 1, Fig. 1) developed prolonged Grade 3 myelotoxicity, lasting for 31/2 weeks. No other nonhematologic or long-term toxicities of normal organs were observed in these patients within a follow-up period of up to 3 years. This held true for the thyroid as well, which is the normal organ with the highest radiation exposure, where no indication for latent or manifest hypothyroidism had yet been obtained at the time this article was written.
|Organ||Radiation absorbed dose (cGy/mCi) (mean ± SD)|
|Whole-body||0.93 ± 0.37|
|Red marrow (blood)a||3.02 ± 1.34|
|Liver||2.47 ± 1.22|
|Spleen||3.16 ± 1.78|
|Lung||2.39 ± 1.27|
|Kidney||3.77 ± 1.34|
|Thyroid||37.1 ± 16.8|
Therapeutic Efficacy in Patients with Known Metastatic Disease
Overall, of 19 assessable patients with radiologically documented metastatic disease, 3 experienced partial remissions (corresponding to an objective response rate of 16%; duration of response, 3–15 months; Table 1). Eight patients (i.e., 42%) experienced minor or mixed responses, or stabilization of their previously rapidly progressing disease, or both, lasting from 3 to 14 months (corresponding to an overall response rate of 58%; mean duration of response, 9 months; range, 3–15 months). Figure 2 shows two examples of objective responses (partial remissions) in patients with liver and lung metastases, respectively.
Interestingly, 9 of the 11 responders were patients with rectal primaries (2 in the colon), in contrast to only 2 of the 8 nonresponders (6 with colonic primaries). Although most lesions larger than 2.5 cm were clearly visualized in the posttherapy gamma camera scans, smaller lesions frequently escaped the scintigraphic detection. Thus, no reliable tumor dosimetry was available in these instances, and no attempt to correlate achieved tumor doses with antitumor effects was made in this study.
Therapeutic Efficacy in an Adjuvant Setting
At the time this article was written, 7 of the 9 patients treated in an adjuvant setting after resection of liver metastases had remained free of disease for up to 36 months (1 patient relapsed after 6, another after 30 months; median observation period, 27 months), whereas the relapse rate in a historical control group (67 patients) who received 5-FU and leucovorin chemotherapy in our hospital according to the Ardalan scheme25 was 67% over the same time frame (Table 2, Fig. 3). Both relapsing patients redeveloped liver metastases, and one also developed two lung lesions. The tumor marker (CEA, CA19-9) levels in the relapse-free patients remained within normal limits.
Five patients with radiologically documented lesions, having experienced at least disease stabilization as a consequence of RIT (one partial response, four minor responses), were retreated at the same 60-mCi/m2 dose level at 8–16 months after the first therapy. No evidence of a potential change in radioantibody pharmacokinetics as a potential indicator of human antihuman antibody formation was observed. Also, no evidence of increased toxicity was obtained: none of the patients developed hematologic toxicity greater than Grade 3, and no evidence of second-organ toxicity was obtained. Of these retreated patients, two of four experienced partial remissions; another of these four again experienced disease stabilization as a consequence of the second radioantibody therapy injection.
Although RIT is an attractive concept for a more target-oriented systemic cancer therapy than is usually achieved with other forms of systemic therapy,10 its major drawback in solid tumors is the problem of achieving sufficiently high tumor uptake, thus sufficient radiation doses.10, 15, 16 This has led to rather disappointing results for bulky, solid tumors.16 We and others have shown previously that tumor uptake and radiation doses to the tumor are inversely correlated with tumor size.15 This has led to the hypothesis that RIT may be a viable therapeutic option for both small-volume metastatic disease and, to an even greater degree, in an adjuvant setting.
Our previous preclinical data suggested that RIT may be therapeutically superior to standard 5-FU and leucovorin, the more “novel” irinotecan chemotherapy, or “cold” (i.e., unlabeled, “naked”) immunotherapeutic approaches.17 Similar data on the comparison of RIT and 5-FU chemotherapy had been reported in several preclinical subcutaneous and metastatic preclinical colon cancer models.26–28 In accordance with these preclinical data, we demonstrated in a clinical Phase I study that the 131I-labeled humanized anti-CEA MAb hMN-14 may provide an encouraging therapeutic efficacy in small-volume colorectal cancer.17, 29 The toxicity in this Phase I study was restricted to transient myelosuppression.17 An objective response rate of almost 20% was observed in these patients, even though most of them had experienced failure with prior chemotherapy. In an additional 45%, previously rapidly progressing disease was stabilized for more than 1 year after RIT.17 Since these results compared very favorably with those of the most successful chemotherapeutic regimens for colorectal cancer, but caused less toxicity, we embarked on further Phase II studies with 131I-hMN-14 in patients with colorectal cancer.
The data from this Phase II study, as presented here, clearly confirm this low-toxicity profile anticipated from the Phase I data. The vast majority of patients experienced Grade 3 myelotoxicity or less. Interestingly, the only patient who developed a Grade 4 thrombocytopenia (in addition to a Grade 3 leukopenia) was the only patient who had previously been exposed to the potent radiosensitizer mitomycin. The radiosensitizing effects of this chemotherapeutic drug and the finding that these effects may last for years after the last mitomycin administration are well known from earlier studies.24
The thyroid is well known as the organ with the highest radiation exposure when radioiodinated immunoconjugates are used.21 We have reported previously on the various factors that may influence the radiation dosimetry to this organ as well as on the finding that, even with high amounts of nonradioactive iodide, complete thyroid blocking is not achievable in most instances.21 This is reflected by mean thyroid radiation doses of approximately 40 cGy/mCi observed in this study. That no hypothyroidism was observed in any of the patients included in this study is, at a 60 mCi/m2 dose level, well in accord with resulting mean thyroid doses of below 50 Gy.
The therapeutic efficacy data from this Phase II study of patients with radiologically documented disease seem to confirm, on a larger scale, the promising results obtained in the previous Phase I trial. An overall response rate of nearly 60% (approximately 20% objective, 40% minor responses) is similar to response rates achieved with 5-FU/leucovorin-based standard chemotherapeutic regimens.30 The observation that patients with rectal primary tumors were more likely to respond than those with colonic primary tumors is most interesting. Retrospectively, the same observation could be made in the previous Phase I study,17 where metastatic disease from rectal primary tumors was much more likely to respond than that from colonic primary tumors. Whether this may reflect a higher CEA expression (and thus potentially higher radioantibody uptake and radiation-absorbed doses), or a higher radiosensitivity of rectal adenocarcinoma as compared with cancers of colonic origin, or another completely unknown effect, must remain speculative at this point.
Although the number of patients treated in an adjuvant setting is much too low for in-depth statistical analyses and/or speculations, the finding that these patients had much better relapse-free survival than a historical control group of patients treated according to the Ardalan protocol25, 30 encourages further adjuvant study protocols. This seems even more important, since adjuvant chemotherapy has failed to show significant effects on the patients' overall and relapse-free survival after resection of surgically treatable liver metastases.1, 6 If these promising data hold true in larger controlled studies, RIT may be the first effective adjuvant treatment modality in this challenging clinical situation.
The data in this communication are based on a “single-shot” treatment. Further studies are necessary in order to determine whether retreatment protocols may improve therapeutic results. The finding that radioantibody readministration did not cause an increased toxicity appears, in this context, especially encouraging.
In summary, our data suggest that RIT may be a viable therapeutic option in colorectal cancer patients with limited disease and especially in an adjuvant setting. Myelotoxicity is the only dose-limiting toxicity. Further studies are ongoing in order to show whether combination approaches of RIT with potentially radiosensitizing chemotherapeutic agents (such as 5-FU, camptothecin, or oxaliplatin derivatives) may further enhance therapeutic efficacy.31 Retreatment regimens seem to be a feasible option. A prospective randomized comparison of RIT with standard chemotherapy is indicated.
The expert technical assistance of Ms. D. Hempel and Ms. M. Pleuger in imaging the patients is gratefully acknowledged by the authors. The authors thank Ms. E. Weber for her help in dosimetry calculations and Prof. Dr. E. Grabbe and Dr. D. Müller for the radiological evaluation of our patients.
- 1DeVita VT, Hellman S, Rosenberg SA, editors. Cancer: principles and practice of oncology. 5th edition. Philadelphia: Lippincott-Raven, 1997.
- 19Evaluation of a complementarity-determining region-grafted (humanized) anti-carcinoembryonic antigen monoclonal antibody in preclinical and clinical studies. Cancer Res 1995; 55: 5935–45., , , , , , et al.
- 22An automated scheme for internal radiation dosimetry in nuclear therapy: gamma- or bremsstrahlung-based dose calculation in the therapy of differentiated thyroid cancer, radiosynoviorthesis, bone pain palliation and radioimmuno-therapy. J Nucl Med 1997; 38: 225P., , , , , .
- 24Hematologic toxicity in the radioimmunotherapy of solid cancers with 131I-labeled anti-CEA NP-4 IgG1: dependence on red marrow dosimetry and pretreatment. Proceedings of the Sixth International Radiopharmaceutical Dosimetry Symposium, Gatlinburg, TN, 1996;1: 113–26., , , , , , et al.