C.H. and K.M. contributed equally to this study.
Evaluation of the proliferation marker MIB-1 in the prognosis of cutaneous malignant melanoma
Article first published online: 23 JUL 2002
Copyright © 2002 American Cancer Society
Volume 95, Issue 3, pages 634–640, 1 August 2002
How to Cite
Hazan, C., Melzer, K., Panageas, K. S., Li, E., Kamino, H., Kopf, A., Cordon-Cardo, C., Osman, I. and Polsky, D. (2002), Evaluation of the proliferation marker MIB-1 in the prognosis of cutaneous malignant melanoma. Cancer, 95: 634–640. doi: 10.1002/cncr.10685
- Issue published online: 23 JUL 2002
- Article first published online: 23 JUL 2002
- Manuscript Accepted: 4 MAR 2002
- Manuscript Revised: 30 JAN 2002
- Manuscript Received: 16 NOV 2001
- American Skin Association
- NIAMS. Grant Number: K08 AR02129
- Manhattan Veterans Affairs Medical Center, New York, NY 10010
The proliferation marker MIB-1, which recognizes the Ki-67 antigen, provides independent prognostic information in several tumor types. Its utility in melanoma has been evaluated mostly in studies of thick primary tumors. Its usefulness in thinner lesions has not been assessed adequately.
A well characterized cohort of 137 patients diagnosed with primary cutaneous melanoma at the New York University School of Medicine between 1972 and 1982 was studied based on the availability of representative tissues and adequate clinical follow-up. Twenty-one tumors were less than or equal to 1.0 mm thick, 94 were between 1.01 and 4.0 mm thick, and 22 were thicker than 4.0 mm. Tumor cell proliferation was assessed by immunohistochemistry using the monoclonal antibody MIB-1. MIB-1 expression was correlated with baseline clinicopathologic parameters, as well as recurrence (RR), disease-free (DFS), and overall survival (OS) rates. Median follow-up among survivors was 6.5 years (range, 5.6–17.5).
High proliferative index, defined as 20% or more of tumor cells showing nuclear immunoreactivity, was observed in 65 of 137 (47.4%) cases. High proliferative index was significantly correlated with increased tumor thickness (P < 0.001) and higher stage (P = 0.03). Trends approaching statistical significance were observed with ulceration of the primary tumor (P = 0.09), male gender (P = 0.06), and shorter DFS (P = 0.12). No significant associations were seen between high proliferative index and RR or OS. In multivariate analyses, tumor thickness was the strongest predictor of clinical outcome.
In primary cutaneous melanoma, a high proliferative index is associated with clinicopathologic parameters predictive of worse clinical outcomes. However, it was not an independent predictor of clinical outcome. Cancer 2002;95:634–40. © 2002 American Cancer Society.