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Characterization of 11 human sarcoma cell strains
Evaluation of cytogenetics, tumorigenicity, metastasis, and production of angiogenic factors
Article first published online: 17 SEP 2002
Copyright © 2002 American Cancer Society
Volume 95, Issue 7, pages 1569–1576, 1 October 2002
How to Cite
Hu, M., Nicolson, G. L., Trent, J. C., Yu, D., Zhang, L., Lang, A., Killary, A., Ellis, L. M., Bucana, C. D. and Pollock, R. E. (2002), Characterization of 11 human sarcoma cell strains. Cancer, 95: 1569–1576. doi: 10.1002/cncr.10879
- Issue published online: 17 SEP 2002
- Article first published online: 17 SEP 2002
- Manuscript Accepted: 30 APR 2002
- Manuscript Revised: 8 APR 2002
- Manuscript Received: 20 JUN 2001
- National Cancer Institute Cancer Center. Grant Number: P30-16672
- Department of Health and Human Services. Grant Numbers: CA 67802, CA 63045, CA 60488, CA74821
- National Institutes of Health Hematology and Oncology. Grant Number: T-32 CA 09666
- cell strain
Human sarcomas have a propensity for aggressive local invasion and early pulmonary metastasis. Frequently, deaths are due to uncontrolled pulmonary metastases. The purpose of the current study was to evaluate cytogenetics, tumorigenicity, metastatic potential, and production of angiogenic factors in human sarcoma cell strains. A secondary purpose was to establish low passage cell strains for studying new therapeutic approaches.
The authors established 11 cell strains from human sarcoma surgical specimens and characterized their in vitro tumor properties, including growth in soft agar, expression of angiogenic growth factors (vascular endothelial growth factor [VEGF] and basic-fibroblast growth factor [bFGF]), and cytogenetics.
All of the cell strains remained diploid. All exhibited the ability to grow in soft agar and expressed both VEGF as well as bFGF. In addition, 6 of the 11 established sarcoma cell strains were tumorigenic, 5 of which spontaneously metastasized to the lungs in nude mice. Four of the five cell strains that yielded lung metastases were derived from lung metastases in patients.
The 11 cell strains, which were derived from diverse sarcoma histologies, will provide a model for studying not only metastatic progression but also the in vitro and in vivo efficacy of new therapeutic modalities for human sarcomas. Cancer 2002;95:1569–76. © 2002 American Cancer Society.