R-Ras promotes tumor growth of cervical epithelial cells
Article first published online: 17 JAN 2003
Copyright © 2003 American Cancer Society
Volume 97, Issue 3, pages 575–585, 1 February 2003
How to Cite
Rincón-Arano, H., Rosales, R., Mora, N., Rodriguez-Castañeda, A. and Rosales, C. (2003), R-Ras promotes tumor growth of cervical epithelial cells. Cancer, 97: 575–585. doi: 10.1002/cncr.11093
- Issue published online: 17 JAN 2003
- Article first published online: 17 JAN 2003
- Manuscript Accepted: 30 AUG 2002
- Manuscript Revised: 23 AUG 2002
- Manuscript Received: 11 JUN 2002
- Consejo Nacional de Ciencia y Tecnología, Mexico. Grant Number: 36407-M
- cervical carcinoma;
- signal transduction;
- cell migration;
R-Ras is 55% identical to H-Ras. However, these two oncogenes seem to have different tumor-transforming potential. R-Ras induced cell transformation in fibroblasts but not in other cell types. R-Ras also reportedly induces a more invasive phenotype in breast epithelial cells through integrin activation. The authors studied the mechanisms whereby R-Ras induces a malignant phenotype.
Dominant negative (R-Ras43N) and constitutively active (R-Ras87L) mutants of R-Ras were stably transfected into human cervical epithelium C33A cells. Transfected cells were analyzed for adhesion, cell spreading, migration, and growth in culture and in nude mice. The activity of extracellular signal-regulated kinase (ERK) and phosphatidylinositol 3-kinase (PI 3-K) also was determined by Western blot analysis and by in vitro kinase assays.
R-Ras87L-transfected cells, but not R-Ras43 N-transfected cells, had a higher growth rate in nude mice and in culture compared with control cells. None of the transfected C33A cells showed an increase in cell adhesion to fibronectin or collagen I, nor did they show an increment of β1 integrin affinity. However, cells that expressed R-Ras87L, but not cells that expressed R-Ras 43N, presented a marked increase in cell spreading and migration through collagen-coated membranes. Increases in cell proliferation, spreading, and migration induced by R-Ras87L were inhibited by the PI 3-K inhibitor LY294002. In addition, PI 3-K activity, but not ERK activity, was increased only in cells that expressed R-Ras87L.
These data suggest that the oncogene R-Ras promotes tumor growth of cervical epithelial cells and increases their migration potential over collagen through a pathway that involves PI 3-K. Cancer 2003;97:575–85. © 2003 American Cancer Society.