The first two authors contributed equally to this work.
Analysis of the Na,K-ATPase α- and β-subunit expression profiles of bladder cancer using tissue microarrays
Article first published online: 1 APR 2003
Copyright © 2003 American Cancer Society
Volume 97, Issue 8, pages 1859–1868, 15 April 2003
How to Cite
Espineda, C., Seligson, D. B., James Ball, W., Rao, J., Palotie, A., Horvath, S., Huang, Y., Shi, T. and Rajasekaran, A. K. (2003), Analysis of the Na,K-ATPase α- and β-subunit expression profiles of bladder cancer using tissue microarrays. Cancer, 97: 1859–1868. doi: 10.1002/cncr.11267
- Issue published online: 1 APR 2003
- Article first published online: 1 APR 2003
- Manuscript Accepted: 9 DEC 2002
- Manuscript Revised: 2 DEC 2002
- Manuscript Received: 15 JUL 2002
- National Institute of Health (NIH). Grant Numbers: DK56216, NIHGMO8243-13
- National Cancer Institute (NCI). Grant Number: NCI1F31CA 93084-01
- sodium pump;
- Na,K-ATPase α-subunit;
- Na,K-ATPase β-subunit;
- bladder cancer;
- tissue microarray
The purpose of this study was to determine the clinical significance of Na,K-ATPase α- and β-subunit expression in a histopathologically well-characterized group of patients representing a wide spectrum of tumor grades and disease stages with transitional cell carcinomas (TCC).
Na,K-ATPase α- and β-subunit protein expression patterns were analyzed using immunohistochemistry on urothelial cancer tissue microarrays (TMA) of 146 patients diagnosed with urothelial carcinoma. For each subunit, the maximum staining intensity and the percentage of positive cells staining at the maximal intensity were analyzed.
Compared with the benign fields, the mean protein expression for both Na,K-ATPase α- and β-subunits were found to be decreased overall in in situ and invasive tumors, as well as in tumor-adjacent dysplastic fields. When Na,K-ATPase α- and β-subunit expression levels were dichotomized into distinct groups, they were both found to be significant predictors of recurrence risk in multivariate logistic regression analysis (P = 0.0062, odds ratio [OR] = 2.6 and P = 0.013, OR = 0.43, for Na,K-ATPase α- and β-subunits, respectively). The authors also found that patients with high α- and low β-subunit expression had a high risk for early recurrence, whereas patients with a low α- and high β-subunit expression had a significantly longer median recurrence-free time (17 months and 125 months, respectively, log rank statistics P = 0.0005).
The results suggested that Na,K-ATPase α- and β-subunit expression levels may be useful predictors of clinical outcomes such as recurrence-free time of bladder cancer patients. Cancer 2003;97:1859–68. © 2003 American Cancer Society.