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Major histocompatibility antigens and antigen-processing molecules in retinoblastoma
Article first published online: 26 JAN 2004
Copyright © 2004 American Cancer Society
Volume 100, Issue 5, pages 1059–1069, 1 March 2004
How to Cite
Krishnakumar, S., Sundaram, A., Abhyankar, D., Krishnamurthy, V., Shanmugam, M. P., Gopal, L., Sharma, T. and Biswas, J. (2004), Major histocompatibility antigens and antigen-processing molecules in retinoblastoma. Cancer, 100: 1059–1069. doi: 10.1002/cncr.20062
- Issue published online: 18 FEB 2004
- Article first published online: 26 JAN 2004
- Manuscript Accepted: 10 DEC 2003
- Manuscript Received: 26 AUG 2003
- Vision Research Foundation (Chennai, India)
- antigen-processing molecules;
- cytotoxic T lymphocytes;
- human leukocyte antigen;
Malignant transformation of cells is frequently associated with abnormalities in human leukocyte antigen (HLA) expression. These abnormalities may play a role in the clinical course of the disease, because HLAs mediate interactions of tumor cells with cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Retinoblastoma is the most common intraocular malignant tumor in childhood and is characterized by direct spread to the optic nerve and orbit as well as hematogeneous and lymphatic spread. Little is known about the role of HLA expression in the progression of this malignant disease.
HLA Class I antigen, β2-microglobulin (β2-m), HLA Class II antigens, and the antigen-processing molecules (APMs) of the HLA Class I pathway, including proteasomal subunits (low–molecular mass polypeptide 2 [LMP-2] and LMP-10), the transporter-associated protein (TAP-1) subunit, the binding protein tapasin, and the chaperone molecule calnexin, were studied in 30 archival retinoblastoma specimens by immunohistochemistry. Immunoanalysis was performed based on the International Histocompatibility Working Group Project Description.
HLA Class I antigen, β2-m, HLA Class II antigen, and APMs were positive in 12 tumors with no invasion and were decreased in 13 tumors with choroidal and optic nerve invasion. The difference in HLA and APM expression between the 2 groups was statistically significant (P < 0.001).
Decreased expression of HLA was observed in aggressive tumors and in poorly differentiated tumors. The current findings support a role for both CTLs and NK cell-mediated control of tumor growth in the clinical course of retinoblastoma. Cancer 2004;100:1059–69. © 2004 American Cancer Society.