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Laser capture microdissection analysis reveals frequent allelic losses in papillary urothelial neoplasm of low malignant potential of the urinary bladder
Version of Record online: 21 MAY 2004
Copyright © 2004 American Cancer Society
Volume 101, Issue 1, pages 183–188, 1 July 2004
How to Cite
Cheng, L., MacLennan, G. T., Zhang, S., Wang, M., Pan, C.-X. and Koch, M. O. (2004), Laser capture microdissection analysis reveals frequent allelic losses in papillary urothelial neoplasm of low malignant potential of the urinary bladder. Cancer, 101: 183–188. doi: 10.1002/cncr.20343
- Issue online: 18 JUN 2004
- Version of Record online: 21 MAY 2004
- Manuscript Revised: 12 APR 2004
- Manuscript Accepted: 12 APR 2004
- Manuscript Received: 10 NOV 2003
- urinary bladder;
- urothelial (transitional cell) carcinoma;
- papillary urothelial neoplasm of low malignant potential;
- loss of heterozygosity;
- laser capture microdissection
In the 1999 World Health Organization classification system, papillary tumors of the urinary bladder were classified as papilloma, papillary urothelial neoplasm of low malignant potential (PUNLMP), and as Grade 1, Grade 2, and Grade 3 urothelial carcinoma. The biologic potential of PUNLMP of the urinary bladder is controversial. To the authors' knowledge, information regarding the genetic changes of PUNLMP tumors of the bladder is limited.
The authors examined loss of heterogygosity (LOH) at 5 polymorphic microsatellite markers on chromosome 9q32–33 (D9S177), 9p22 (IFNA), 17p13.1 (TP53), 12q14–24 (D12S1051), and 3p25–26 (D3S3050) from 26 patients who were diagnosed with PUNLMP tumors of the urinary bladder. Tumors were microdissected from sections prepared from formalin-fixed, paraffin-processed tissue specimens using laser capture microdissection.
LOH was found in 21 of 26 (81%) patients with PUNLMP. The rate of LOH was 41% with D9S177, 32% with IFNA, 29% with TP53, 26% with D12S1051, and 44% with D3S3050. Allelic loss of multiple chromosome loci was often present in patients with PUNLMP tumors.
Genetic changes that commonly occur in advanced bladder carcinoma (≥ pT2) are frequently found in PUNLMP of the urinary bladder. Cancer 2004. © 2004 American Cancer Society.