Fax: (951) 827-5504
Human chorionic gonadotropin β (HCGβ) down-regulates E-cadherin and promotes human prostate carcinoma cell migration and invasion
Article first published online: 1 DEC 2005
Copyright © 2005 American Cancer Society
Volume 106, Issue 1, pages 68–78, 1 January 2006
How to Cite
Wu, W. and Walker, A. M. (2006), Human chorionic gonadotropin β (HCGβ) down-regulates E-cadherin and promotes human prostate carcinoma cell migration and invasion. Cancer, 106: 68–78. doi: 10.1002/cncr.21549
- Issue published online: 23 DEC 2005
- Article first published online: 1 DEC 2005
- Manuscript Accepted: 19 JUL 2005
- Manuscript Revised: 23 JUN 2005
- Manuscript Received: 11 APR 2005
- National Institutes of Health (NIH). Grant Number: DK 61005
- human chorionic gonadotropin β (HCGβ);
- cell migration;
- prostate carcinoma;
- tumor invasion;
Membrane-associated human chorionic gonadotropin β (HCGβ) is correlated with a poor prognosis in localized prostate adenocarcinoma. The relationship between HCGβ and metastasis, however, is unclear.
To shed some light on the issue, two stable prostate carcinoma cell lines overexpressing HCGβ, designated DU145 HCGβ and PC3 HCGβ, were created and compared with empty vector stably transfected DU145 and PC3 cells (control cells).
HCGβ expression resulted in a change in morphology; the cells were more elongated and had multiple pseudopodia, while the control cells were more rounded. This change in morphology was duplicated by incubating control cells in conditioned medium from the DU145 HCGβ or PC3 HCGβ cells, or by adding purified HCGβ to control medium. The DU145 HCGβ and PC3 HCGβ cells were also less adherent than the controls, as assessed by the ease with which trypsin-EDTA could remove them from culture plates. Reduced adherence could be duplicated by incubation of control cells with either conditioned medium or purified HCGβ. Western blot analysis showed that DU145 HCGβ and PC3 HCGβ cells expressed less E-cadherin than control cells and that a change of medium increased expression of E-cadherin. Addition of conditioned medium, or purified HCGβ, to control cells down-regulated E-cadherin. Cell migration and invasion assays showed that DU145 HCGβ and PC3 HCGβ cells were more migratory and invasive than controls and that treatment of control cells with either conditioned medium or purified HCGβ increased their migratory/invasive capacity.
The data indicate that HCGβ is directly responsible for changes in prostate carcinoma cells associated with an increased metastatic phenotype. Cancer 2006. © 2005 American Cancer Society.