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Down-regulation of locus-specific human lymphocyte antigen class I expression in Epstein–Barr virus-associated gastric cancer
Implication for viral-induced immune evasion
Version of Record online: 15 MAR 2006
Copyright © 2006 American Cancer Society
Volume 106, Issue 8, pages 1685–1693, 15 April 2006
How to Cite
Dutta, N., Gupta, A., Mazumder, D. N. G. and Banerjee, S. (2006), Down-regulation of locus-specific human lymphocyte antigen class I expression in Epstein–Barr virus-associated gastric cancer. Cancer, 106: 1685–1693. doi: 10.1002/cncr.21784
- Issue online: 4 APR 2006
- Version of Record online: 15 MAR 2006
- Manuscript Accepted: 4 NOV 2005
- Manuscript Revised: 7 SEP 2005
- Manuscript Received: 6 JUL 2005
- cytotoxic T-lymphocytes;
- gastric cancer;
- Epstein–Barr virus;
- human lymphocyte antigen class I expression
To understand whether the association between Epstein–Barr Virus (EBV) and gastric cancer (GC) has any role in loss of surface expression of human lymphocyte antigen (HLA) class I, the authors analyzed locus-specific transcriptional expression of HLA-A, HLA-B, HLA-C, and HLA-E along with other HLA-associated molecules (β2-microglobulin [β2M], cellular latent membrane protein [LMP], and transporter associated with antigen presentation [TAP]) in EBV-associated, primary GC (EBVaGC) and EBV-negative GC (EBVnGC) tissues.
Approximately 20 EBVaGC tissues and 40 EBVnGC tissues and their corresponding normal tissues were used in the study. The presence of EBV in GC was established by EBV-encoded small RNA in situ hybridization analysis and BamHI W polymerase chain reaction (PCR) analysis. Transcriptional expression of viral LMP2A and several HLA class I genes were analyzed by reverse transcriptase (RT)-PCR. Surface expression levels of HLA class I proteins in cancer samples along with their normal counterparts also were quantified by flow cytometry.
The RT-PCR data suggested selective down-regulation of the HLA-A/HLA-B locus along with over-expression of HLA-E transcripts in EBVaGC (P < .05). This was confirmed further by the flow-cytometric studies using antibodies to HLA-ABC and HLA-E. Among the accessory molecules, LMP7 transcript was down-regulated in a number of EBVaGC tissues compared with EBVnGC.
The current results suggested that the establishment of EBV latent infection in gastric tissues allows malignant cells to avoid the immune surveillance of both cytotoxic T-lymphocytes and natural killer cells by regulating the differential expression of HLA class I molecules. Cancer 2006. © 2006 American Cancer Society.