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Predictive value of p53 and pRb expression in superficial bladder cancer patients treated with BCG and interferon-alpha†
Article first published online: 20 FEB 2007
Copyright © 2007 American Cancer Society
Volume 109, Issue 6, pages 1097–1105, 15 March 2007
How to Cite
Esuvaranathan, K., Chiong, E., Thamboo, T. P., Chan, Y. H., Kamaraj, R., Mahendran, R. and Teh, M. (2007), Predictive value of p53 and pRb expression in superficial bladder cancer patients treated with BCG and interferon-alpha. Cancer, 109: 1097–1105. doi: 10.1002/cncr.22503
All patients in the study gave written informed consent.
- Issue published online: 7 MAR 2007
- Article first published online: 20 FEB 2007
- Manuscript Accepted: 27 NOV 2006
- Manuscript Revised: 13 NOV 2006
- Manuscript Received: 29 AUG 2006
- The National Medical Research Council (Singapore). Grant Number: NMRC/0085/1995/NMRC/0457/2000
- bladder cancer;
- bacille Calmette Guerin vaccine;
- p53 gene;
- retinoblastoma gene
Nuclear p53 and retinoblastoma protein (pRb) were reported to be poor prognostic indicators for transitional cell carcinoma of the bladder. The authors sought to determine the prognostic value of nuclear p53 and pRb in superficial bladder transitional cell carcinoma patients who were treated with intravesical bacille Calmette-Guerin (BCG) or BCG with interferon-alpha (IFN-α).
A prospective histological review was performed for 80 superficial bladder transitional cell carcinoma patients who underwent postresection intravesical regimes of BCG (81mg, n = 33 or 27 mg, n = 20) or BCG (27 mg) with IFN-α (n = 27), and followed for a mean of 4.5 years. Hematoxylin and eosin (H & E) and immunoperoxidase staining were performed on tissue sections. Nuclear p53 and pRb immunoreactivity were assessed semiquantitatively, by using a combination of staining extent and intensity, to categorize overexpression or underexpression. Data were analyzed by using chi-square analysis, multiple logistic regression, and Kaplan-Meier curves.
pRb expression was not associated with patient outcome after BCG-alone therapy, but pRb underexpression was significantly associated with BCG nonresponse and tumor recurrence (P = .047) after BCG and IFN-α (BCG + IFN-α) therapy. Low-grade tumors were associated with pRb overexpression, with or without nuclear p53 underexpression (P = .019; P = .043, respectively). p53 expression alone or in combination with pRb expression had no significant relation with tumor response to BCG alone or BCG + IFN-α with respect to recurrence, progression, or cancer-specific death.
Nuclear pRb underexpression may be predictive of nonresponse and cancer recurrence after intravesical BCG + IFN-α therapy. Nuclear p53 expression or its combination with pRb expression is not associated with post-BCG clinical outcome, so p53 expression or p53 with pRb expression should not be used to influence decisions concerning BCG-alone or BCG + IFN-α therapy. Cancer 2007. © 2007 American Cancer Society.