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Risk assessment of renal cell carcinoma using alkaline comet assay
Article first published online: 4 JUN 2007
Copyright © 2007 American Cancer Society
Volume 110, Issue 2, pages 282–288, 15 July 2007
How to Cite
Lin, X., Wood, C. G., Shao, L., Huang, M., Yang, H., Dinney, C. P. and Wu, X. (2007), Risk assessment of renal cell carcinoma using alkaline comet assay. Cancer, 110: 282–288. doi: 10.1002/cncr.22792
- Issue published online: 29 JUN 2007
- Article first published online: 4 JUN 2007
- Manuscript Accepted: 28 FEB 2007
- Manuscript Revised: 27 FEB 2007
- Manuscript Received: 20 NOV 2006
- National Cancer Institute. Grant Number: RO1 CA098897
- risk assessment;
- renal cell carcinoma;
- alkaline comet assay;
DNA damage induced by mutagens has been associated with an individual's susceptibility to cancer.
In the current study, which involved 193 renal cell carcinoma (RCC) patients and 193 controls, DNA damage before mutagen induction (baseline), after benzo(α)pyrene dio epoxide (BPDE) treatment, and after γ-radiation induction were assayed by comet assay in peripheral blood lymphocytes. Olive tail moments were used as DNA damage parameters. The 5 variables that were analyzed for their associations with RCC risk were baseline, BPDE-induced, γ-radiation-induced, net BPDE-induced (BPDE-induced subtract baseline), and net γ-radiation-induced (γ-radiation-induced subtract baseline) Olive tail moments.
Significantly higher Olive tail moments were observed in cases compared with controls at baseline (1.95 vs 1.65; P = .008), after BPDE induction (3.10 vs 2.38; P < .001), and after γ-radiation induction (4.25 vs 3.47; P < .001). The net BPDE-induced and γ-radiation-induced DNA damage was also found to be significantly higher in cases compared with controls (P < .001 for both mutagens). Using the 75th percentile Olive tail moments in the controls as the cutoff point, the authors found that high levels of baseline DNA damage, BPDE-induced DNA damage, and γ-radiation-induced DNA damage were associated with significantly increased risks of RCC, with odds ratios of 1.96 (95% confidence interval [95% CI], 1.26–3.06), 2.70 (95% CI, 1.72–4.23), and 3.13 (95% CI, 1.99–4.92), respectively. Similarly, net BPDE-induced and net γ-radiation-induced DNA damages were also found to be significantly associated with elevated risks of RCC.
The results of the current study suggest that both baseline and mutagen-induced DNA damages assessed by comet assay are associated with an increased risk of RCC. Cancer 2007. © 2007 American Cancer Society.