Fax: (770) 488-4639.
Article first published online: 3 NOV 2008
Published 2008 by the American Cancer Society
Supplement: Assessing the Burden of HPV-Associated Cancers in the United States
Volume 113, Issue Supplement 10, pages 2901–2909, 15 November 2008
How to Cite
Ryerson, A. B., Peters, E. S., Coughlin, S. S., Chen, V. W., Gillison, M. L., Reichman, M. E., Wu, X., Chaturvedi, A. K. and Kawaoka, K. (2008), Burden of potentially human papillomavirus-associated cancers of the oropharynx and oral cavity in the US, 1998–2003. Cancer, 113: 2901–2909. doi: 10.1002/cncr.23745
This article is a US Government work and, as such, is in the public domain in the United States of America.
We appreciate the in-kind support from all the contributors to this supplement. We also thank Amy Chen, MD, MPH, for her input on the categorization of anatomic sites for this analysis.
The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.
Fax: (770) 488-4639.
- Issue published online: 3 NOV 2008
- Article first published online: 3 NOV 2008
- Manuscript Accepted: 2 JUN 2008
- Manuscript Revised: 29 MAY 2008
- Manuscript Received: 14 APR 2008
- Cooperative Agreement. Grant Number: U50 DP424071-04
- Centers for Disease Control and Prevention (CDC)
- human papillomavirus;
- oral cancer;
- oropharyngeal cancer
As human papillomavirus (HPV) vaccination becomes widely available in the US for cervical cancer prevention, it may also affect the rates of other cancers potentially associated with HPV. The objective of the current study was to describe the incidence rates of oropharyngeal and oral cavity cancers in the US with a focus on anatomic sites potentially associated with HPV infection.
Incident cases diagnosed between 1998 and 2003 identified through 39 population-based registries that participate in the National Program of Cancer Registries and/or the Surveillance, Epidemiology, and End Results Program were examined. The incidence rates of potentially HPV-associated oropharyngeal and oral cavity cancers by various characteristics were estimated. The 1998 through 2003 trends in these rates were also compared with rates for sites not previously shown to be associated with HPV (comparison sites).
In all, 44,160 cases of potentially HPV-associated cancers of the oropharynx and oral cavity were identified, including 19,239 (43.6%) tonsillar, 16,964 (38.4%) base of tongue, and 7957 (18.0%) other oropharyngeal cancers. The incidence rates for these sites were highest among blacks, and higher among non-Hispanics and men than among Hispanics and women. The annual incidence rates of potentially HPV-associated cancers of the tonsil and base of tongue both increased significantly from 1998 through 2003 (annual percentage change [APC], 3.0; P < .05 for both sites), whereas the incidence rates of cancer at the comparison sites generally decreased.
The results of the current study provide baseline incidence rates of potentially HPV-associated cancers of the oropharynx and oral cavity that can be compared with rates after the widespread implementation of the HPV vaccination. Cancer 2008;113(10 suppl):2901–9. Published 2008 by the American Cancer Society.
The anatomy of the pharynx (oropharynx, hypopharynx, and nasopharynx) and oral cavity (lip, tongue, gum, floor of mouth, and palate) is a complex region. In a recent study, researchers estimated that 34,360 new cases of pharyngeal and oral cavity cancer would be diagnosed in 2007 in the US and that 7550 people will die from these cancers.1 The incidence rates for these cancers are higher among men and blacks than among women and whites.2 The mortality rate for these cancers is also significantly higher among black men (6.9 per 100,000) than among white men (3.7 per 100,000).2 Greater than 90% of the cancers in this anatomic region are squamous cell carcinomas.3, 4
The most frequently cited risk factors for cancers of the pharynx and oral cavity are tobacco and alcohol use, and the combined effect on cancer risk is believed to be multiplicative rather than additive.5–8 Although most of these cancers in developed countries have been attributed to these risk factors,3 some patients report having never smoked or drank heavily.9, 10 Recent epidemiologic and experimental data provide evidence for an association between human papillomavirus (HPV) infection and a subset of oropharyngeal and oral cavity cancers.7, 11–23 This evidence includes a positive association between risk for oropharyngeal cancer and lifetime number of vaginal and/or oral sex partners.23
There is considerable variation in the proportion of cancers that are HPV-positive among the different sites of the oropharynx and oral cavity.7, 11, 12, 14–19, 21, 22, 24, 25 In a review of 60 studies, Kreimer et al24 calculated the average HPV-DNA positivity rate to be 35.6% for oropharyngeal carcinomas and 23.5% for oral cavity carcinomas. Overall, approximately 25% of these cancers have been associated with high-risk HPV types, with HPV-16 accounting for 87% of HPV-infected oropharyngeal cancers and 68% of HPV-infected oral cavity carcinomas.24, 26–29 Among the cancers of the oropharynx and oral cavity, tonsillar squamous cell carcinoma has been most strongly and consistently associated with HPV-16 infection.16, 19, 30, 31
The recognition that HPV plays any role in oropharyngeal and oral cavity cancer has important implications for cancer prevention. As the vaccination for HPV becomes widely available in the US for cervical cancer prevention, the incidence rates of other cancers potentially associated with HPV, such as those of the oropharynx and oral cavity, may also decline. To provide baseline data against which to measure the effect of the HPV vaccine on rates of these cancers, we estimated the incidence rates of squamous cell carcinoma of the oropharynx and oral cavity in the US from 1998 through 2003, with a focus on anatomic sites in these areas previously found to be associated with HPV infection.
MATERIALS AND METHODS
Cases included in this analysis were microscopically confirmed, newly diagnosed in situ or invasive squamous cell carcinomas (International Classification of Disease for Oncology, 3rd edition [ICD-O-3] morphology codes 8050-8084, 8120-8131) of the oropharynx and oral cavity identified from population-based cancer registries that participated in either the National Program of Cancer Registries (NPCR); the Surveillance, Epidemiology, and End Results (SEER) program; or both from 1998 through 2003.2, 32 NPCR and SEER data from 38 states and the District of Columbia met the data quality criteria (US Cancer Statistics Publication Standard) for all cancer sites during this period and were included in this analysis.
We focused our analysis on oropharynx and oral cavity carcinomas most strongly associated with HPV infection. Because the NPCR and the SEER Program do not collect information regarding the presence of HPV within tumors, we relied exclusively on anatomic sites' proclivity for HPV reported in previous studies.7, 11, 12, 14–19, 21, 22, 24, 25, 33–35 We divided potentially HPV-associated sites into 3 major anatomic areas: the tonsil, including the Waldeyer ring; the base of the tongue and the lingual tonsil; and other potentially HPV-associated sites within the oropharynx. Detailed descriptions and specific ICD-O-3 topography codes used to define these unique anatomic groupings are shown in Table 1.
|Sites of Potentially HPV-Associated Cancers|
|Tonsil (including Waldeyer ring)|
|C09.0 Tonsillar fossa|
|C09.1 Tonsillar pillar|
|C09.8 Overlapping lesion of tonsil|
|C09.9 Tonsil, NOS|
|C14.2 Waldeyer ring|
|Base of tongue and lingual tonsil|
|C01.9 Base of tongue, NOS|
|C02.4 Lingual tonsil|
|Other oropharynx sites|
|C02.8 Overlapping lesion of tongue|
|C10.2 Lateral wall of oropharynx|
|C10.8 Overlapping lesion of oropharynx|
|C10.9 Oropharynx, NOS|
|C14.0 Pharynx, NOS|
|C14.8 Overlapping lesion of lip, oral cavity and pharynx|
|C02.0 Dorsal surface of tongue, NOS|
|C02.1 Border of tongue|
|C02.2 Ventral surface of tongue, NOS|
|C02.3 Anterior 2/3 of tongue, NOS|
|C02.9 Tongue, NOS|
|Other oral cavity sites|
|C03.0 Upper gum|
|C03.1 Lower gum|
|C03.9 Gum, NOS|
|C04.0 Anterior floor of mouth|
|C04.1 Lateral floor of mouth|
|C04.8 Overlapping lesion of floor of mouth|
|C04.9 Floor of mouth, NOS|
|C05.0 Hard palate|
|C06.0 Cheek mucosa|
|C06.1 Vestibule of mouth|
|C06.2 Retromolar area|
|C06.8 Overlapping lesion of other and unspecified parts of mouth|
|C06.9 Mouth, NOS|
|C32.3 Laryngeal cartilage|
|C32.8 Overlapping lesion of larynx|
|C32.9 Larynx, NOS|
|Other oropharynx sites|
|C05.1 Soft palate, NOS|
|C05.8 Overlapping lesion of palate|
|C05.9 Palate, NOS|
|C10.1 Anterior surface of epiglottis|
|C10.3 Posterior wall of oropharynx|
We examined incidence rates for in situ and invasive potentially HPV-associated oropharyngeal and oral cavity cancers, and examined invasive cases independently by cancer site and stage at diagnosis and by the age, sex, race, ethnicity, and geographic region of the study subjects. Because of potential racial misclassification, information regarding American Indian and Alaska Natives was not available for this analysis.36 Rates were calculated per 100,000 people and adjusted by direct standardization to the 2000 US population. SEER-Stat software (version 6.1.4) was used to calculate counts, age-adjusted rates, and 95% confidence intervals (95% CIs).37 The stages of cancer cases diagnosed from 1998 through 2000 were classified according to the 1977 SEER summary staging system38 criteria, whereas the stages of cases diagnosed from 2001 through 2003 were classified according to the 2000 SEER summary staging system39 criteria. Although changes between the 2 systems did result in differences in how regional and distant stage at some sites were classified, we accounted for these differences by defining SEER localized cancers as early-stage disease and combining SEER regional and distant cancers into a single category of late-stage disease.40, 41
Lastly, we included a subanalysis of incidence rates of cancers that have not been previously associated with HPV infection but are anatomically similar to the potentially HPV-associated cancers (hereafter referred to as comparison sites). We divided the comparison sites into 4 major anatomic areas (Table 1): the tongue, other oral cavity sites, other sites within the oropharynx, and a subset of sites within the larynx that are within close proximity to several potentially HPV-associated sites (eg, glottis, supraglottis, and subglottis). We examined trends in incidence rates for the comparison sites by year of diagnosis to compare trends among the potentially HPV-associated cancers. We calculated the annual percent change (APC) in incidence rates from 1998 through 2003 by fitting a least-squares regression line to the natural logarithm of the rates, using the calendar year as a regression variable. We rejected the hypothesis that the APC equaled 0 if the P was ≤0.05.
Incidence Rates of Potentially HPV-associated Invasive Oropharyngeal and Oral Cavity Cancers
The study data file for 1998 through 2003 contained 44,160 cases of potentially HPV-associated invasive oropharyngeal and oral cavity cancers, of which 19,239 (43.6%) were tonsillar, 16,964 (38.4%) were base of tongue, and 7957 (18.0%) were other potentially HPV-associated oropharyngeal cancers (Table 2). The age-adjusted incidence rates were at least 3 times higher among men than women for all site categories. The incidence rates were also higher among blacks than other racial groups, higher among non-Hispanics than among Hispanics, and generally higher among those who lived in the South. In addition, most of these cases were diagnosed at a late stage (78.1% for tonsil, 77.4% for base of tongue, and 68.7% for other oropharynx cancers). Compared with cancers of the tonsil and base of tongue and lingual tonsil, other oropharyngeal cancers were more likely to occur in older people, women, and blacks, and to have been diagnosed at an earlier stage.
|Characteristic||Tonsil||Base of Tongue and Lingual Tonsil||Other Oropharynx|
|No.||%||Rate||(95% CI)||No.||%||Rate||(95% CI)||No.||%||Rate||(95% CI)|
|Age group (median)||(57)||(60)||(63)|
|Asian or Pacific Islander||259||1.3||0.5||(0.4-0.6)||186||1.1||0.4||(0.3-0.4)||77||1.0||0.2||(0.1-0.2)|
Incidence Rates of Potentially HPV-associated Invasive Tonsil Cancers by Sex and Race/Ethnicity
Incidence rates of tonsil cancer among men stratified by age and race/ethnicity were highest among blacks ages 60 to 69 years (9.0; 95% CI, 8.1-10.0), and lowest among Asian or Pacific Islander (API) men of all ages (Fig. 1). The rates of tonsillar cancer among women were substantially lower than those among men and were highest among women ages 60 to 69 years (2.0%; 95% CI, 1.9-2.1%) and 70 to 79 years (2.0%; 95% CI, 1.9-2.2).
Incidence Rates of Potentially HPV-associated Invasive Base of Tongue Cancers by Sex and Race/Ethnicity
Incidence rates of base of tongue carcinomas among men stratified by age and race/ethnicity were also highest among blacks ages 60 to 69 years (9.5; 95% CI, 8.6-10.5), whereas API men had the lowest rate among men in this age group (2.1; 95% CI, 1.5-2.9) (Fig. 2). Overall, among men ages 40 to 79 years, the rates were highest among blacks, whereas among men aged ≥80 years, the rates were highest among whites and Hispanics. Women again had substantially lower rates than men, with the incidence rate highest among those ages 70 to 79 years. Although rates among women differed less by race/ethnicity than rates among men, black women ages 40 to 59 years did have a higher rate of invasive base of tongue carcinomas than white or Hispanic women in the same age group.
Incidence Rates of Other Potentially HPV-associated Oropharyngeal Cancers by Sex and Race/Ethnicity
The incidence rates of potentially HPV-associated carcinomas at other oropharyngeal sites were lower than those observed for tonsil or base of tongue cancers among both men and women (Fig. 3). Similar to the other cancers, however, the incidence rates for other oropharyngeal cancers were highest among men ages 60 to 79 years, with the highest rate noted among black men ages 60 to 69 years (7.7; 95% CI, 6.9-8.6). Among women, the rate was highest among those ages 70 to 79 years (1.4; 95% CI, 1.3-1.5), and rates differed little by race or ethnicity.
Average Annual Number of Potentially HPV-associated Oropharyngeal and Oral Cavity Cancers and Comparison Sites
An average of 7360 cases of potentially HPV-associated invasive oropharyngeal and oral cavity cancers were diagnosed annually from 1998 through 2003 (Fig. 4). Overall, only 1.1% of the potentially HPV-associated cases were diagnosed in situ, whereas 5.4% of the comparison cases were.
Trends in Incidence Rates of Potentially HPV-associated Invasive Oropharyngeal and Oral Cavity Cancers and Comparison Sites
The age-adjusted cancer incidence rates of tonsillar and base of tongue cancers increased significantly for men and women combined from 1998 through 2003 (APC, 3.0; P < .05 for both), whereas rates declined significantly during this period at almost all the comparison sites (Fig. 5). Laryngeal cancer incidence rates declined most sharply from 4.5 (95% CI, 4.4-4.6) in 1998 to 3.7 (95% CI, 3.7-3.8) in 2003, an APC of −3.5. Among men (Fig. 6), the rates of tonsil and base of tongue cancers increased significantly (P < .05), whereas the rate of laryngeal cancers declined (APC, −3.6; P < .05). Among women (Fig. 7), the incidence rates remained fairly stable at potentially HPV-associated sites but increased marginally at most of the comparison sites.
The results of the current study support prior findings demonstrating that cancer incidence rates at sites within the pharynx and oral cavity were higher among blacks than other racial/ethnic groups and higher among men than women.1 These disparities in incidence rates could be due, at least in part, to differences in rates of high-risk HPV infection or to differences in the prevalence and intensity of exposure to other risk factors such as smoking or alcohol consumption. Historical data from the 1994-1995 National Health Interview Survey showed that the age-adjusted prevalence of current smoking was 18.0% among Hispanics, 26.4% among whites, and 26.5% among blacks, and that in all these racial and ethnic groups men had significantly higher rates of current smoking than woman.42 Comparable alcohol consumption prevalence data were unavailable.
Differences in rates of early diagnosis and treatment of precursor lesions among various subpopulations may also be a factor in the observed differences, although to our knowledge data to support this hypothesis are limited. In addition, in situ disease for all these cancers is rare because the premalignant lesions in these anatomic areas are largely asymptomatic and are difficult to detect visually. However, several studies have shown that people with lower income or educational attainment have an increased risk for cancers of the pharynx and oral cavity.43, 44
We were particularly concerned about the apparent increase in rates of cancers of the tonsil and base of tongue in a relatively short time period. In a recent analysis of SEER data from 1973 through 2004, Chaturvedi et al45 reported an increase in incidence rates for HPV-associated oral squamous cell carcinomas. Similar trends in incidence rates, particularly for tonsil and tongue carcinomas, have been observed previously.46, 47 Also recently, Sturgis and Cinciripini48 suggested that increases in rates of cancer in certain sites of the oropharynx, despite the dramatic reduction in tobacco exposure among Americans over the past 40 years, could be attributable to HPV infections.
This study was limited by several factors. The first involved the difficulty of determining which cancers were associated with HPV given that 1) cancer of the oropharynx and oral cavity can involve multiple adjacent sites without clear indications of where the primary tumor may have originated, 2) previously reported associations between these cancers and HPV varied substantially by subsite, and 3) these associations were generally much weaker than in other regions, such as the cervix. Because we were unable to classify the HPV status of a tumor on the basis of HPV detection, we relied on anatomic associations presented in the literature. Although multiple lines of molecular and epidemiologic evidence suggest that HPV may have an etiologic role in the development of oropharyngeal and oral cavity cancer, much of the data were based on tumor HPV detection alone, and the proportion of these cancers found to be associated with HPV has varied. This variation has been attributed in part to differences in sampling techniques and HPV detection methods.21 However, in a recent study based on data from the International Agency for Research on Cancer (IARC) multinational study, Parkin and Bray49 estimated HPV-attributable fractions of 12% for oropharyngeal cancer and 3% for oral cancer, which were substantially lower than the percentage of tumors with detectable HPV DNA.11, 14–16, 18, 22, 25, 49 Because of these factors, we limited our analysis to sites such as the tonsil and base of tongue, in which HPV has been found to be associated with all spectrums of disease.33–35 Viral copy numbers for some tumors at these sites have been comparable to those reported in other HPV-associated malignancies, such as cervical cancer.50 However, despite limiting our analysis in this way, some of the cancer cases in our analyses may have been misclassified because of the complexity of determining primary cancer sites in this anatomic region.
A second limitation to the current study was that because central cancer registries do not collect data on tobacco or alcohol use among cancer cases, we were unable to assess the extent to which these risk factors may have affected the association between HPV infection and cancer incidence rates. To establish a baseline measurement in this dataset against which to measure the future impact of the HPV vaccine on potentially HPV-associated cancers of this type, we conducted subanalyses of cancer incidence rates at anatomically similar sites within the oropharynx, oral cavity, and larynx that to our knowledge have not been previously associated with HPV infection. The results of these subanalyses may help future researchers determine the potential effectiveness of HPV vaccination in preventing oropharyngeal and oral cavity cancers over time. However, it is important to note that none of the vaccine trials have assessed the efficacy of the HPV vaccine in reducing the incidence rate of oropharyngeal and oral cavity HPV infection; therefore, more studies are needed to determine the effect that the vaccine will have on incidence rates of these cancers.
Cancers of the oral tongue, oral cavity, and larynx that are unrelated to HPV and likely attributable primarily to tobacco and alcohol use are more than twice as frequent as cancers of the oropharynx and oral cavity potentially associated with HPV, and, as noted above, the HPV vaccination has not been shown to prevent oropharyngeal and oral cavity cancers. Nevertheless, with an average of 7360 cases of potentially HPV-associated cancers occurring annually in the US, the morbidity and death associated with these cancers is still substantial. Thus, measures to reduce the risk of infection with high-risk HPV strains (including HPV vaccination and sexual risk reduction), combined with avoidance of smoking and excessive alcohol use, could potentially prevent thousands of cases of cancer of the oropharynx and oral cavity in the US each year.
- 2U.S. Cancer Statistics Working Group. U.S. cancer statistics: 2004 incidence and mortality. Atlanta, GA: Department of Health and Human Services, Centers for Disease Control and Prevention and National Cancer Institute; 2007.
- 37Surveillance Research Program. National Cancer Institute SEER-Stat Software, Version 6.1.4. Bethesda, MD: National Cancer Institute; 2005.
- 38Surveillance, Epidemiology, and End Results Program. Summary staging guide for the Cancer Surveillance, Epidemiology, and End Results (SEER) Program. Bethesda, MD: National Institutes of Health, National Cancer Institute; 1977
- 39Surveillance, Epidemiology, and End Results Program. Summary staging manual 2000. Bethesda, MD: National Institutes of Health, National Cancer Institute; 2005.
- 40http://www.naaccr.org/index.asp?Col_SectionKey=11&Col_ContentID=397. Accessed on July 11, 2008., , , , . Site-specific comparison of summary stage 1977 and summary stage 2000 coding. Available from URL:
- 41PhillipsJL, ed. Summary stage: data effects of the changes in 2000. Available from URL: http://www.naaccr.org/filesystem/pdf/Summary%20Stage%20Report%201-21-04b.pdf. Accessed on July 11, 2008.
- 42Department of Health and Human Services. Tobacco use among U.S. racial/ethnic minority groups—African-Americans, American-Indians and Alaska Natives, Asian-Americans and Pacific Islanders, and Hispanics: a report of the Surgeon General. Atlanta, GA: Centers for Disease Control and Prevention; 1998.