Epithelial and pseudoepithelial differentiation in glioblastoma and gliosarcoma

A comparative morphologic and molecular genetic study

Authors

  • Fausto J. Rodriguez MD,

    Corresponding author
    1. Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, Minnesota
    • Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, 200 First Street SW, Mayo Clinic, Rochester, MN 55905===

    Search for more papers by this author
    • Fax: (507) 284-1599.

  • Bernd W. Scheithauer MD,

    1. Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, Minnesota
    Search for more papers by this author
  • Caterina Giannini MD, PhD,

    1. Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, Minnesota
    Search for more papers by this author
  • Sandra C. Bryant MS,

    1. Department of Health Sciences Research, Division of Biostatistics, Mayo Clinic College of Medicine, Rochester, Minnesota
    Search for more papers by this author
  • Robert B. Jenkins MD, PhD

    1. Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, Minnesota
    Search for more papers by this author

Abstract

BACKGROUND.

Glioblastomas exhibit a remarkable tendency toward morphologic diversity. Although rare, pseudoepithelial components (adenoid or epithelioid) or true epithelial differentiation may occur, posing a significant diagnostic challenge.

METHODS.

Hematoxylin and eosin–stained slides were reviewed, and immunohistochemistry and fluorescence in situ hybridization were performed.

RESULTS.

The patients included 38 men and 20 women. The median age at diagnosis was 57 years (interquartile range [IQR], 50 years-67 years), and the median overall survival was 7 months (IQR, 4 months-11 months). “Adenoid” glioblastomas (A-GBM) predominated (48%). True epithelial glioblastomas (TE-GBM) were next most frequent based on morphology and immunohistochemistry (35%), followed by epithelioid glioblastomas (E-GBM) (17%). Overall, 25 (43%) tumors featured a sarcomatous component. Molecular cytogenetic abnormalities identified by fluorescent in situ hybridization in A-GBM, E-GBM, and TE-GBM, respectively, included p16 deletion/-9 (60%, 71%, 64%); chromosome 10 loss (40%, 63%, 57%), chromosome 7 gain without EGFR amplification (70%, 38%, 40%), EGFR amplification (10%, 50%, 27%), PTEN deletion (10%, 25%, 29%), PDGFRA amplification (10%, 25%, 0%), and RB1 deletion/−13q (50%, 0%, 14%). Abnormalities identified by immunohistochemistry included p21 immunonegativity (60%, 25%, 93%), which was most frequent in TE-GBM (P = .008), strong nuclear p53 staining (29%, 29%, 41%), strong membranous staining for epidermal growth factor receptor (EGFR) (21%, 63%, 19%), which was most frequent in E-GBM (P = .03), and an increased frequency of p27 immunonegativity in gliosarcomas (15% negative, 85% focal) compared with tumors without sarcoma (38% strongly positive) (P = .009).

CONCLUSIONS.

Pseudoepithelial and true epithelial morphology are rare phenomena in GBM and may be associated with a similar poor prognosis. These tumors demonstrate proportions of molecular genetic abnormalities varying somewhat from conventional GBM. Cancer 2008. © 2008 American Cancer Society.

Ancillary