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Flavonoids activated caspases for apoptosis in human glioblastoma T98G and U87MG cells but not in human normal astrocytes
Article first published online: 5 NOV 2009
Copyright © 2010 American Cancer Society
Volume 116, Issue 1, pages 164–176, 1 January 2010
How to Cite
Das, A., Banik, N. L. and Ray, S. K. (2010), Flavonoids activated caspases for apoptosis in human glioblastoma T98G and U87MG cells but not in human normal astrocytes. Cancer, 116: 164–176. doi: 10.1002/cncr.24699
- Issue published online: 11 JAN 2010
- Article first published online: 5 NOV 2009
- Manuscript Accepted: 10 APR 2009
- Manuscript Revised: 2 APR 2009
- Manuscript Received: 6 FEB 2009
- National Institutes of Health. Grant Numbers: CA-91460, NS-57811
Human glioblastoma is a deadly brain cancer that continues to defy all current therapeutic strategies. The authors induced apoptosis in human glioblastoma T98G and U87MG cells after treatment with apigenin, (−)-epigallocatechin, (−)-epigallocatechin-3-gallate (EGCG), and genistein, which did not induce apoptosis in human normal astrocytes.
Induction of apoptosis was examined using Wright staining and ApopTag assay. Production of reactive oxygen species (ROS) and increase in intracellular free Ca2+ were measured by fluorescent probes. Analysis of mRNA and Western blotting indicated increases in expression and activities of the stress kinases and cysteine proteases for apoptosis. JC-1 showed changes in mitochondrial membrane potential (ΔΨm), and use of specific inhibitors confirmed activation of kinases and proteases in apoptosis.
Treatment of glioblastoma cells with apigenin, (−)-epigallocatechin, EGCG, or genistein triggered ROS production that induced apoptosis with phosphorylation of p38 mitogen-activated protein kinase (MAPK) and activation of the redox-sensitive c-Jun N-terminal kinase 1 pathway. Pretreatment of cells with ascorbic acid attenuated ROS production and p38 MAPK phosphorylation. Increases in intracellular free Ca2+ and activation of caspase-4 indicated involvement of endoplasmic reticulum stress in apoptosis. Other events in apoptosis included overexpression of Bax, loss of ΔΨm, mitochondrial release of cytochrome c and Smac into the cytosol, down-regulation of baculoviral inhibitor-of-apoptosis repeat-containing proteins, and activation of calpain, caspase-9, and caspase-3. (−)-Epigallocatechin and EGCG also induced caspase-8 activity. Apigenin, (−)-epigallocatechin, EGCG, and genistein did not induce apoptosis in human normal astrocytes.
Results strongly suggest that flavonoids are potential therapeutic agents for induction of apoptosis in human glioblastoma cells. Cancer 2010. © 2010 American Cancer Society.