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Radiofrequency field-induced thermal cytotoxicity in cancer cells treated with fluorescent nanoparticles†
Version of Record online: 19 APR 2010
Copyright © 2010 American Cancer Society
Volume 116, Issue 13, pages 3285–3293, 1 July 2010
How to Cite
Glazer, E. S. and Curley, S. A. (2010), Radiofrequency field-induced thermal cytotoxicity in cancer cells treated with fluorescent nanoparticles. Cancer, 116: 3285–3293. doi: 10.1002/cncr.25135
We thank Dr. Paul Cherukuri for assistance in discussion of nanoparticle properties, and Kristine Ash, Katheryn Massey, and Katrina Briggs for administrative assistance and cell culture assistance.
- Issue online: 18 JUN 2010
- Version of Record online: 19 APR 2010
- Manuscript Accepted: 3 NOV 2009
- Manuscript Received: 22 SEP 2009
- quantum dot;
- noninvasive radiofrequency field treatment;
- photothermal therapy;
- hyperthermic cytotoxicity
Nonionizing radiation, such as radiofrequency field and near infrared laser, induces thermal cytotoxicity in cancer cells treated with gold nanoparticles. Quantum dots are fluorescent semiconducting nanoparticles that were hypothesized to induce similar injury after radiofrequency field irradiation.
Gold nanoparticles and 2 types of quantum dot (cadmium-selenide and indium-gallium-phosphide) conjugated to cetuximab (C225), a monoclonal antibody against human epidermal growth factor receptor (EGFR)-1, demonstrated concentration-dependent heating in a radiofrequency field. The authors investigated the effect of radiofrequency field exposure after targeted nanoparticle treatment in a coculture of 2 human cancer cell lines that have differential EGFR-1 expression (a high-expressing pancreatic carcinoma, Panc-1, and a low-expressing breast carcinoma, Cama-1).
Radiofrequency revealed that Panc-1 or Cama-1 cells not containing gold nanoparticles or quantum dots had a viability of >92%. The viability of Panc-1 cells exposed to the radiofrequency field after treatment with 50 nM Au-C225 was 39.4% ± 8.3% without injury to bystander Cama-1 cells (viability was 93.7% ± 1.0%; P ∼ .0006). Panc-1 cells treated with targeted cadmium-selenide quantum dots were only 47.5% viable after radiofrequency field exposure (P<.0001 compared with radiofrequency only Panc-1 control cells). Targeted indium-gallium-phosphide quantum dots decreased Panc-1 viability to 58.2% ± 3.4% after radiofrequency field exposure (P = ∼.0004 compared with Cama-1 and Panc-1 controls).
The authors selectively induced radiofrequency field cytotoxicity in Panc-1 cells without injury to bystander Cama-1 cells using EGFR-1–targeted nanoparticles, and demonstrated an interesting bifunctionality of fluorescent nanoparticles as agents for both cancer cell imaging and treatment. Cancer 2010. © 2010 American Cancer Society.