Relation between normal rectal methylation, smoking status, and the presence or absence of colorectal adenomas

Authors

  • Bogdan C. Paun MD,

    1. Division of Gastroenterology, Department of Medicine, the Johns Hopkins University School of Medicine, Baltimore, Maryland
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    • The first two authors contributed equally to this article.

  • Debra Kukuruga MD,

    1. Department of Pathology, University of Maryland School of Medicine, Baltimore, Maryland
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    • The first two authors contributed equally to this article.

  • Zhe Jin MD, PhD,

    1. Division of Gastroenterology, Department of Medicine, the Johns Hopkins University School of Medicine, Baltimore, Maryland
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  • Yuriko Mori MD,

    1. Division of Gastroenterology, Department of Medicine, the Johns Hopkins University School of Medicine, Baltimore, Maryland
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  • Yulan Cheng MD,

    1. Division of Gastroenterology, Department of Medicine, the Johns Hopkins University School of Medicine, Baltimore, Maryland
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  • Mark Duncan MD,

    1. Department of Surgery, the Johns Hopkins University School of Medicine, Baltimore, Maryland
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  • Sanford A. Stass MD,

    1. Department of Pathology, University of Maryland School of Medicine, Baltimore, Maryland
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  • Elizabeth Montgomery MD,

    1. Department of Pathology, the Johns Hopkins University School of Medicine, Baltimore, Maryland
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  • David Hutcheon MD,

    1. Division of Gastroenterology, Department of Medicine, the Johns Hopkins University School of Medicine, Baltimore, Maryland
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  • Stephen J. Meltzer MD

    Corresponding author
    1. Division of Gastroenterology, Department of Medicine, the Johns Hopkins University School of Medicine, Baltimore, Maryland
    2. Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, the Johns Hopkins University School of Medicine, Baltimore, Maryland
    • Department of Oncology, Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University, 1503 E. Jefferson Street, Room 112, Baltimore, MD 21287
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    • Fax: (410) 502-1329


Abstract

BACKGROUND:

Colorectal cancer (CRC) is 1 of the leading causes of death in the Western world. CRC develops from premalignant lesions, chiefly colorectal adenomas. Currently, the most accurate and recommended screening method for finding colorectal adenomas is colonoscopy performed on all individuals aged >50 years. However, the costs and risks associated with this procedure are relatively high. The objectives of the current study were to correlate epigenetic alterations that occur in normal rectal mucosa, smoking status, and age with the presence or absence of concomitant colorectal adenomas and to assess the potential clinical value of methylation in normal rectal biopsies as a screening assay for the presence of polyps and, hence, the need for a full colonoscopy.

METHODS:

One hundred thirteen normal rectal mucosal biopsies from 113 patients were studied. DNA was extracted, modified with sodium bisulfite, and subjected to real-time quantitative, methylation-specific polymerase chain reaction analysis for the following genes: adenomatous polyposis coli (APC); cadherin 1, type 1, E-cadherin (epithelial) (CDH1); estrogen receptor 1 (ESR1); cytokine high in normal 1 (HIN1); hyperplastic polyposis protein 1 (HPP1); O-6 methylguanine-DNA methyltransferase (MGMT); neural epidermal growth factor-like 1 (NELL1); splicing factor 3B, 14-kDa subunit (p14); cyclin-dependent kinase (CDK) inhibitor 2B (inhibits CDK4) (p15); retinoic acid receptor beta (RARβ); somatostatin (SST); tachykinin, precursor 1 (TAC1); and tissue inhibitor of metalloproteinase (TIMP) metallopeptidase inhibitor 3 (TIMP3). Data were then analyzed using several proprietary software programs.

RESULTS:

By using several sets of genes, clinical characteristics, and multivariate analyses, the authors developed a prediction model for the presence of concomitant colorectal adenomas at the time of rectal biopsy. They also observed strong correlations between smoking status and rectal methylation pattern and between smoking status and the presence or risk of concomitant adenomas.

CONCLUSIONS:

A prediction model was developed for the presence of colorectal adenomas based on gene methylation patterns in the normal rectum. The results indicated that these genes may be involved in early stages of adenoma formation. The observed epigenetic alterations in these markers may be caused in part by the effects of smoking and/or age. Normal rectal methylation may be useful as a biomarker for narrowing the population in need of screening colonoscopy. Cancer 2010. © 2010 American Cancer Society.

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