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Demonstration of oncogenic properties in pancreatic cancer
Article first published online: 30 SEP 2010
Copyright © 2010 American Cancer Society
Volume 117, Issue 4, pages 723–733, 15 February 2011
How to Cite
Liu, S.-H., Patel, S., Gingras, M.-C., Nemunaitis, J., Zhou, G., Chen, C., Li, M., Fisher, W., Gibbs, R. and Brunicardi, F. C. (2011), PDX-1. Cancer, 117: 723–733. doi: 10.1002/cncr.25629
- Issue published online: 3 FEB 2011
- Article first published online: 30 SEP 2010
- Manuscript Accepted: 6 AUG 2010
- Manuscript Received: 26 JUL 2010
- transcription factor;
- pancreatic cancer;
Pancreatic-duodenal homeobox 1 (PDX-1) is a transcription factor that regulates embryologic pancreas development and insulin expression in the adult islet; however, it is overexpressed in many types of cancer, including pancreatic cancer. The purpose of this study was to investigate the role of PDX-1 in tumorigenesis in human cells.
In vitro cell proliferation, invasion, and transformation were performed in human embryonic kidney cell line (HEK 293), pancreatic cancer cell line MIA PaCa2, and human pancreatic ductal epithelial (HPDE) cells transiently or stably expressing PDX-1 or green fluorescent protein (GFP) PDX-1, with or without cotransfection of PDX-1 short hairpin RNA (shRNA). In vivo tumor formation was carried out in severe combined immunodeficiency (SCID) mice with subcutaneous injection of HEK 293 and MIA PaCa2 stably transfected cells. Cell cycle was analyzed by Western blot or immunostaining. Microarray of RNA from pancreatic adenocarcinoma cells with and without PDX-1 shRNA was performed and analyzed.
Transient and stable expressing PDX-1 significantly increased cell proliferation and invasion in HEK 293, human pancreatic ductal epithelial (HPDE), and MIA PaCa2 cells versus controls (P < .05), human PDX-1 shRNA reversed these effects. Expression of PDX-1 significantly increased colony formation in HEK 293, HPDE, and MIA PaCa2 cells versus controls in vitro (P < .05). PDX-1 promoted HEK 293 and MIA PaCa2 tumor formation in SCID mice as compared with that of control (P < .05). PDX-1 overexpression disrupted cell cycles proteins. PDX-1 expression was confirmed by Western blot and tracked by viewing of GFP–PDX-1 expression. Microarray data support an oncogenic role of PDX-1 in pancreas cancer cells.
PDX-1 induced increased cell proliferation, invasion, and colony formation in vitro, and resulted in markedly increased HEK 293 and MIA PaCa2 tumor formation in SCID mice. These data suggest that PDX-1 is a potential oncogene that regulates tumorigenesis. Cancer 2011. © 2010 American Cancer Society.