Profiling of Epstein-Barr virus-encoded microRNAs in nasopharyngeal carcinoma reveals potential biomarkers and oncomirs
Article first published online: 1 FEB 2012
Copyright © 2011 American Cancer Society
Volume 118, Issue 18, page 4634, 15 September 2012
How to Cite
Gourzones, C., Jimenez, A.-S. and Busson, P. (2012), Profiling of Epstein-Barr virus-encoded microRNAs in nasopharyngeal carcinoma reveals potential biomarkers and oncomirs. Cancer, 118: 4634. doi: 10.1002/cncr.26514
- Issue published online: 5 SEP 2012
- Article first published online: 1 FEB 2012
In an article published in Cancer ahead of print on June 30, 2011, Wong et al report the distinct presence of several viral microRNAs encoded by the Epstein-Barr virus (EBV) genome in the serum from patients bearing EBV-associated nasopharyngeal carcinomas (NPCs).1 These findings open exciting perspectives for use of viral microRNAs as new tools for early NPC diagnosis, prognosis assessment, and treatment monitoring. The report by Wong et al is in part convergent with our previously published data about the detection of the EBV microRNA BART7 in plasma samples from patients with NPC.2 However, there are differences.
In contrast to Wong et al, we have observed a low but significant level of BART7 amplification from non-NPC donor samples. The primers used for reverse transcription and polymerase chain reaction were identical in both studies, but 1 obvious difference in our protocol was that RNA was extracted from plasma instead of from serum samples. We are curious to know whether, according to the authors, RNA extraction from serum samples is a better option to increase specific detection of circulating microRNA-BARTs among patients with NPC. Another potential factor of inconsistency is the use of a preamplification step after retrotranscription of RNAs. Wong et al refer to a protocol described by Kroh et al that mentions this preamplification procedure as optional.3 In our own study, we have skipped this step; however, it would be interesting to know explicitly whether or not it has been performed by Wong et al.
- 1Profiling of Epstein-Barr virus-encoded microRNAs in nasopharyngeal carcinoma reveals potential biomarkers and oncomirs [published online ahead of print June 30, 2011]. Cancer. 2011; 118: 698–710, , , , .
- 2Extra-cellular release and blood diffusion of BART viral micro-RNAs produced by EBV-infected nasopharyngeal carcinoma cells [serial online]. Virol J. 2010; 7: 271., , , et al.
- 3Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-PCR (qRT-PCR). Methods. 2010; 50: 298-301., , , .
Claire Gourzones PhD*, Anne-Sophie Jimenez*, Pierre Busson MD, PhD*, * CNRS and Gustave Roussy Institute, University of Paris, Villejuif, France.